Ago2 Antibody, Brachyury Antibody, Brn3A Antibody, Cd45Ro Antibody, Cdk5 Antibody, Erg Antibody, Foxa2 Antibody, Gerbil, Goat, Guinea, Hamster, Horse, Human, Insect, Jmjd3 Antibody, Kangaroo, Killifish, Klf2 Antibody, Mobp Antibody, Parp1 Antibody, Pig, Rabbit, Raccoon, Rat, Reindeer, Tbp Antibody, Zeb2 Antibody
Modified microassay for the isolation of antimicrobial-producing, spore-forming and nonspore-forming bacteria
OBJECTIVE
To develop a microassay technique to detect antimicrobials produced by spore-forming micro organism, thus dashing up the invention of recent antimicrobials.
RESULTS
Environmental isolates had been grown in 96-well plates, to permit manufacturing of antimicrobial brokers, then handled with lysozyme and heated sequentially. Lysozyme warmth therapy inhibited or prevented spore-to-cell transformation, thus eliminating interference from spore outgrowth whereas detecting antimicrobials by indicator micro organism. Supplementation of the indicator pressure medium with 2,3,5-triphenyltetrazolium chloride, as a significant stain, made it straightforward to quickly differentiate between antimicrobial-deficient (indicator progress) and antimicrobial-containing (no progress) wells.
The tactic was used to quickly display screen 657 micro organism remoted from eight soil samples. Outcomes revealed 46 Bacillus sp. producing antimicrobials in opposition to Listeria sp., and a Bacillus sp. producing antimicrobial(s) in opposition to Escherichia coli.
CONCLUSIONS
A microassay technique was efficiently developed and applied to display screen and detect antimicrobial brokers from spore-forming, along with nonspore-forming, micro organism.
CONCLUSIONS
Antimicrobials are wanted to fight antibiotic- and preservative-resistant micro organism. Spore-forming micro organism are prolific producers of antimicrobials. This assay will pace the invention of antimicrobials from spore-forming micro organism; these new antimicrobials are urgently wanted in meals and medicinal functions.
Microassay for ketamine and metabolites in plasma and serum based mostly on enantioselective capillary electrophoresis with extremely sulfated γ-cyclodextrin and electrokinetic analyte injection
For the evaluation of stereoselective points of the metabolism of ketamine, an enantioselective CE-based microassay for dedication of the stereoisomers of ketamine and three of its main metabolites in plasma and serum was developed. The assay is predicated on liquid/liquid extraction of the analytes of curiosity at alkaline pH from 0.05 mL plasma or serum adopted by electrokinetic pattern injection of the analytes from the extract throughout a buffer plug with out chiral selector.
Separation happens cationically at regular polarity in a pH 3.Zero phosphate buffer containing 0.66% of extremely sulfated γ-cyclodextrin (HS-γ-CD). Key parameters for optimization are recognized as being the quantity of HS-γ-CD within the BGE, the size of the buffer plug and its focus, the period of electrokinetic injection, and the extraction medium. Diluted buffer within the plug is employed to determine adequate analyte stacking as a consequence of a mixture of discipline amplification and complexation.
The newly developed microassay is strong (intraday and interday RSD < 5% and <9%, respectively) and properly suited to find out enantiomer ranges of ketamine and its metabolites all the way down to 10 ng/mL. It’s extra delicate, makes use of much less plasma or serum, natural solvent, and evaluation time in comparison with earlier CE-based assays and was efficiently utilized to watch ketamine, norketamine, 5,6-dehydronorketamine (DHNK), and 6-hydroxynorketamine (6HNK) stereoisomer ranges in plasma of a Beagle canine that obtained a bolus of racemic ketamine or S-ketamine after sevoflurane anesthesia. The info recommend that the formation of DHNK and 6HNK happen stereoselectively.

Discovery and microassay of a nitrite-dependent carbonic anhydrase exercise by stable-isotope dilution gasoline chromatography-mass spectrometry
The intrinsic exercise of carbonic anhydrase (CA) is the hydration of CO2 to carbonic acid and its dehydration to CO2. CA may additionally perform as esterase and phosphatase. Just lately, we demonstrated that renal CA is especially liable for the reabsorption of nitrite (NO2(-)) which is probably the most plentiful reservoir of the biologically extremely potent nitric oxide (NO). Via a stable-isotope dilution GC-MS technique, we found a novel CA exercise which strictly relies upon upon nitrite. We discovered that bovine erythrocytic CAII (beCAII) catalyses the incorporation of (18)O from H2 (18)O into nitrite at pH 7.4.
After derivatization with pentafluorobenzyl bromide, gasoline chromatographic separation and mass spectrometric evaluation, we detected ions at m/z 48 for singly (18)O-labelled nitrite ((16)O=N-(18)O(-)/(18)O=N-(16)O(-)) and at m/z 50 for doubly (18)O-labelled nitrite ((18)O=N-(18)O(-)) along with m/z 46 for unlabelled nitrite. Utilizing (15)N-labelled nitrite ((15)NO2 (-), m/z 47) as an inside commonplace and selected-ion monitoring of m/z 46, m/z 48, m/z 50 and m/z 47, we developed a GC-MS microassay for the quantitative dedication of the nitrite-dependent beCAII exercise.
The CA inhibitors acetazolamide and FC5 207A didn’t alter beCAII-catalysed formation of singly and doubly (18)O-labelled nitrite. Cysteine and the experimental CA inhibitor DIDS (a diisothiocyanate) elevated a number of fold the beCAII-catalysed formation of the (18)O-labelled nitrite species. Cysteine, acetazolamide, FC5 207A, and DIDS by themselves had no impact on the incorporation of (18)O from H2 (18)O into nitrite. We conclude that erythrocytic CA possesses a nitrite-dependent exercise which may solely be detected when nitrite is used because the substrate and the response is carried out in buffers of impartial pH values ready in H2 (18)O.
This novel CA exercise, i.e., the nitrous acid anhydrase exercise, represents a bioactivation of nitrite and will have each useful (by way of S-nitrosylation and subsequent NO launch) and presumably hostile (by way of C- and N-nitrosylation) results in residing organisms.
![]() Recombinant Human Galectin-1 Protein |
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PROTP09382-1 | BosterBio | 50ug | EUR 380.4 |
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues. |
![]() Anti-Galectin-3 Monoclonal Antibody |
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M00621-1 | BosterBio | 100ul | EUR 476.4 |
Description: Mouse Monoclonal Galectin-3 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
![]() Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
![]() anti-Galectin 1 |
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YF-PA12945 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Galectin 1 |
![]() HRP-Goat Anti-Mouse Secondary Antibody |
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A12003 | EpiGentek |
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![]() HRP-Goat Anti-Rabbit Secondary Antibody |
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A12004 | EpiGentek |
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![]() Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated |
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A12001 | EpiGentek |
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![]() Goat Anti-Rat Secondary Antibody, Biotin Conjugated |
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A12002 | EpiGentek |
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![]() Anti-Galectin 1 Antibody |
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A00470-2 | BosterBio | 100ug/vial | EUR 352.8 |
![]() Anti-Galectin-1 antibody |
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PAab03314 | Lifescience Market | 100 ug | EUR 426 |
![]() anti- Galectin-1 antibody |
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FNab03314 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Galectin-1 |
![]() anti- Galectin-1 antibody |
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FNab03315 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Galectin-1 |
![]() Anti-Galectin-1 antibody |
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STJ93201 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-1. |
![]() Anti-Galectin-1 antibody |
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STJ98716 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-1. |
![]() Anti-Galectin 1 (1A8) |
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YF-MA13984 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to Galectin 1 |
![]() Human Galectin-1 (LGALS1) |
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1-CSB-EP012882HU | Cusabio |
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Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli |
![]() Human Galectin-1 (LGALS1) |
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1-CSB-EP012882HUb1 | Cusabio |
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Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli |
![]() Human Genomic DNA |
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X11000 | EpiGentek |
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![]() Anti-Galectin 1/Lgals1 Antibody |
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A00470 | BosterBio | 100ug/vial | EUR 400.8 |
![]() Anti-Galectin 1 Biotinylated Antibody |
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A00470-Biotin | BosterBio | 50ug/vial | EUR 352.8 |
![]() anti-Galectin 1 (1E8-1B2) |
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LF-MA10174 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to Galectin 1 |
![]() Anti-Galectin 1/LGALS1 Antibody |
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PA1422 | BosterBio | 100ug/vial | EUR 400.8 |
![]() Anti-Galectin 1/LGALS1 Antibody |
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PB9240 | BosterBio | 100ug/vial | EUR 400.8 |
![]() Anti-galectin-1 (mouse) antibody |
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STJ72519 | St John's Laboratory | 100 µg | EUR 430.8 |
![]() Human Brain Genomic DNA |
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X11001 | EpiGentek |
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![]() Anti-Galectin-13 (GAL13) / Placental Protein 13 (PP13) Monoclonal Antibody |
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M08143-1 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Mouse Monoclonal Galectin-13 (GAL13) / Placental Protein 13 (PP13) Antibody. Validated in IHC and tested in Human. |
![]() Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
![]() Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
![]() LGALS8 Human, Galectin-8 Human Recombinant Protein, His Tag |
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PROTO00214-1 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: LGALS8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37.9 kDa. The LGALS8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
![]() LGALS3 Human, Galectin-3 Human Recombinant Protein, His Tag |
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PROTP17931-1 | BosterBio | Regular: 25ug | EUR 380.4 |
Description: LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 270 amino acids (1-250 a.a.) and having a molecular mass of 28.3 kDa._x000D_ The LGALS3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. _x000D_ |
![]() LGALS7 Human, Galectin-7 Human Recombinant Protein, His Tag |
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PROTP47929-1 | BosterBio | Regular: 20ug | EUR 380.4 |
Description: Galectin-7 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids (1-136 a.a.) and having a molecular mass of 17.2kDa. ;The Galectin-7 is purified by proprietary chromatographic techniques. |
![]() Human Galectin-3 (Gal-3) AssayMax ELISA Kit |
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EG3311-1 | AssayPro | 96 Well Plate | EUR 572.4 |
![]() Human Galectin-4 (Gal-4) AssayMax ELISA Kit |
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EG3312-1 | AssayPro | 96 Well Plate | EUR 572.4 |
![]() anti-Galectin 3 |
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YF-PA24079 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Galectin 3 |
![]() anti-galectin 9 |
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YF-PA24081 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to galectin 9 |
![]() anti-Galectin 3 |
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YF-PA12946 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to Galectin 3 |
![]() anti-Galectin 3 |
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YF-PA12947 | Abfrontier | 100 ul | EUR 483.6 |
Description: Rabbit polyclonal to Galectin 3 |
![]() anti-Galectin 3 |
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YF-PA12948 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Galectin 3 |
![]() anti-Galectin 8 |
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YF-PA12951 | Abfrontier | 50 ul | EUR 435.6 |
Description: Mouse polyclonal to Galectin 8 |
![]() anti-Galectin 13 |
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YF-PA18585 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to Galectin 13 |
![]() Human Galectin-1(LGALS1) ELISA kit |
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1-CSB-EL012882HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1(LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
![]() Recombinant Human Galectin-1 |
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7-00427 | CHI Scientific | 10µg | Ask for price |
![]() Recombinant Human Galectin-1 |
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7-00428 | CHI Scientific | 50µg | Ask for price |
![]() Recombinant Human Galectin-1 |
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7-00429 | CHI Scientific | 1mg | Ask for price |
![]() Human Galectin 1 Protein |
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20-abx066725 | Abbexa |
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![]() Galectin-1, human recombinant |
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4648-10 | Biovision | each | EUR 170.4 |
![]() Galectin-1, human recombinant |
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4648-1000 | Biovision | each | EUR 1977.6 |
![]() Galectin-1, human recombinant |
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4648-50 | Biovision | each | EUR 314.4 |
![]() Anti-human Galectin-3 antibody |
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STJ15100163 | St John's Laboratory | 250 µg | EUR 403.2 |
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation |
![]() Bovine Galectin-1 (LGALS1) |
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1-CSB-EP012882BO | Cusabio |
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Description: Recombinant Bovine Galectin-1(LGALS1) expressed in E.coli |
![]() SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag |
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E80025 | EpiGentek |
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![]() Anti-Galectin-9 Antibody |
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A03415 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Galectin-9 Antibody (LGALS9) detection.tested for WB in Human, Mouse, Rat. |
![]() Anti-Galectin 2 antibody |
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PAab03310 | Lifescience Market | 100 ug | EUR 494.4 |
![]() Anti-Galectin 4 antibody |
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PAab03311 | Lifescience Market | 100 ug | EUR 426 |
![]() Anti-Galectin 8 antibody |
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PAab03312 | Lifescience Market | 100 ug | EUR 426 |
![]() Anti-Galectin 9 antibody |
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PAab03313 | Lifescience Market | 100 ug | EUR 426 |
![]() Anti-Galectin-3 antibody |
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PAab03316 | Lifescience Market | 100 ug | EUR 426 |
![]() Anti-Galectin-7 antibody |
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PAab03318 | Lifescience Market | 100 ug | EUR 426 |
![]() anti-Galectin-3 (6G2) |
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LF-MA20336 | Abfrontier | 100 ug | EUR 424.8 |
Description: Mouse monoclonal to Galectin-3 |
![]() anti- Galectin 2 antibody |
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FNab03310 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against Galectin 2 |
![]() anti- Galectin 4 antibody |
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FNab03311 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Galectin 4 |
![]() anti- Galectin 8 antibody |
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FNab03312 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Galectin 8 |
![]() anti- Galectin 9 antibody |
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FNab03313 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Galectin 9 |
![]() anti- Galectin-3 antibody |
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FNab03316 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Galectin-3 |
![]() anti- Galectin-3 antibody |
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FNab03317 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Galectin-3 |
![]() anti- Galectin-7 antibody |
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FNab03318 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Galectin-7 |
![]() Anti-Galectin-2 antibody |
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STJ93202 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-2. |
![]() Anti-Galectin-4 antibody |
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STJ93203 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-4. |
![]() Anti-Galectin-7 antibody |
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STJ93204 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-7. |
![]() Anti-Galectin-8 antibody |
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STJ93205 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-8. |
![]() Anti-Galectin-9 antibody |
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STJ93206 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Galectin-9. |
![]() Anti-Galectin-3 antibody |
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STJ96945 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Galectin-3 is a protein encoded by the LGALS3 gene which is approximately 26,1 kDa. Galectin-3 is localised to the cytoplasm and nucleus. It is involved in NF-kappaB signalling, advanced glycosylation end product receptor signalling and cell adhesion. It is a galactose-specific lectin which binds IgE and may mediate the stimulation by CSPG4 of endothelial cells migration along with the alpha-3, beta-1 integrin. It is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. Galectin-3 is expressed mainly in the colonic epithelium and is also abundantly expressed in activated macrophages. Mutations in the LGALS3 gene may result in follicular adenoma and thyroid cancer. STJ96945 was developed from clone 6G2 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous galectin-3 proteins. |
![]() Anti-Galectin-3 antibody |
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STJ97549 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3 (6B8). |
![]() Anti-Galectin-3 antibody |
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STJ97550 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3 (8D7). |
![]() Anti-Galectin-3 antibody |
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STJ97551 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3 (5D9). |
![]() Anti-Galectin-3 antibody |
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STJ97601 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3 (2F9). |
![]() Anti-Galectin-3 antibody |
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STJ97604 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3 (16E6). |
![]() Anti-Galectin-3 antibody |
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STJ97605 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3 (1H4). |
![]() Anti-Galectin-3 antibody |
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STJ97715 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to Galectin-3. |
![]() Anti-Galectin-3 antibody |
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STJ16100369 | St John's Laboratory | 1 mL | EUR 1256.4 |
![]() Anti-Galectin-3 antibody |
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STJ16100782 | St John's Laboratory | 100 µg | EUR 864 |
![]() Anti-Galectin-3 antibody |
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STJ180106 | St John's Laboratory | 0.1 ml | EUR 254.4 |
![]() Anti-Galectin 3 antibody |
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STJ190057 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Mouse monoclonal to Galectin 3 (AS1A24) |
![]() Anti-Galectin 3 antibody |
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STJ73098 | St John's Laboratory | 100 µg | EUR 430.8 |
![]() Anti-Galectin 8 (3E5) |
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YF-MA13986 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to Galectin 8 |
![]() Galectin 1 antibody |
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70R-11939 | Fitzgerald | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal Galectin 1 antibody |
![]() Galectin 1 antibody |
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70R-49997 | Fitzgerald | 100 ul | EUR 292.8 |
Description: Purified Polyclonal Galectin 1 antibody |
![]() Galectin 1 antibody |
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70R-13963 | Fitzgerald | 100 ug | EUR 386.4 |
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody |
![]() Galectin 1 protein |
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30R-1385 | Fitzgerald | 100 ug | EUR 268.8 |
Description: Purified recombinant Human Galectin 1 protein |
![]() Galectin 1 protein |
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30R-2371 | Fitzgerald | 50 ug | EUR 423.6 |
Description: Purified recombinant Human Galectin 1 protein |
![]() Galectin 1 Antibody |
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49673-100ul | SAB | 100ul | EUR 399.6 |
![]() Galectin 1 Antibody |
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49673-50ul | SAB | 50ul | EUR 286.8 |
![]() Galectin 1 protein |
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30R-AG003 | Fitzgerald | 10 ug | EUR 159.6 |
Description: Purified recombinant Human Galectin 1 protein |
![]() Galectin-1 Protein |
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20-abx263505 | Abbexa |
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![]() Galectin-1 Protein |
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20-abx261634 | Abbexa |
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![]() Galectin 1 antibody |
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70R-GR002 | Fitzgerald | 100 ug | EUR 571.2 |
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody |
![]() rHu Galectin-1 |
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AK8281-0010 | Akron Biotech | 10µg | Ask for price |
![]() rHu Galectin-1 |
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AK8281-0050 | Akron Biotech | 50µg | Ask for price |
![]() rHu Galectin-1 |
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AK8281-0100 | Akron Biotech | 100µg | Ask for price |
![]() rHu Galectin-1 |
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AK8281-1000 | Akron Biotech | 1mg | Ask for price |