Ago2 Antibody, Brachyury Antibody, Brn3A Antibody, Cd45Ro Antibody, Cdk5 Antibody, Erg Antibody, Foxa2 Antibody, Gerbil, Goat, Guinea, Hamster, Horse, Human, Insect, Jmjd3 Antibody, Kangaroo, Killifish, Klf2 Antibody, Mobp Antibody, Parp1 Antibody, Pig, Rabbit, Raccoon, Rat, Reindeer, Tbp Antibody, Zeb2 Antibody

Modified microassay for the isolation of antimicrobial-producing, spore-forming and nonspore-forming bacteria

by Frank Green
OBJECTIVE
To develop a microassay technique to detect antimicrobials produced by spore-forming micro organism, thus dashing up the invention of recent antimicrobials.
RESULTS
Environmental isolates had been grown in 96-well plates, to permit manufacturing of antimicrobial brokers, then handled with lysozyme and heated sequentially. Lysozyme warmth therapy inhibited or prevented spore-to-cell transformation, thus eliminating interference from spore outgrowth whereas detecting antimicrobials by indicator micro organism. Supplementation of the indicator pressure medium with 2,3,5-triphenyltetrazolium chloride, as a significant stain, made it straightforward to quickly differentiate between antimicrobial-deficient (indicator progress) and antimicrobial-containing (no progress) wells.
The tactic was used to quickly display screen 657 micro organism remoted from eight soil samples. Outcomes revealed 46 Bacillus sp. producing antimicrobials in opposition to Listeria sp., and a Bacillus sp. producing antimicrobial(s) in opposition to Escherichia coli.
CONCLUSIONS
A microassay technique was efficiently developed and applied to display screen and detect antimicrobial brokers from spore-forming, along with nonspore-forming, micro organism.
CONCLUSIONS
Antimicrobials are wanted to fight antibiotic- and preservative-resistant micro organism. Spore-forming micro organism are prolific producers of antimicrobials. This assay will pace the invention of antimicrobials from spore-forming micro organism; these new antimicrobials are urgently wanted in meals and medicinal functions.

Microassay for ketamine and metabolites in plasma and serum based mostly on enantioselective capillary electrophoresis with extremely sulfated γ-cyclodextrin and electrokinetic analyte injection

For the evaluation of stereoselective points of the metabolism of ketamine, an enantioselective CE-based microassay for dedication of the stereoisomers of ketamine and three of its main metabolites in plasma and serum was developed. The assay is predicated on liquid/liquid extraction of the analytes of curiosity at alkaline pH from 0.05 mL plasma or serum adopted by electrokinetic pattern injection of the analytes from the extract throughout a buffer plug with out chiral selector.
Separation happens cationically at regular polarity in a pH 3.Zero phosphate buffer containing 0.66% of extremely sulfated γ-cyclodextrin (HS-γ-CD). Key parameters for optimization are recognized as being the quantity of HS-γ-CD within the BGE, the size of the buffer plug and its focus, the period of electrokinetic injection, and the extraction medium. Diluted buffer within the plug is employed to determine adequate analyte stacking as a consequence of a mixture of discipline amplification and complexation.
The newly developed microassay is strong (intraday and interday RSD < 5% and <9%, respectively) and properly suited to find out enantiomer ranges of ketamine and its metabolites all the way down to 10 ng/mL. It’s extra delicate, makes use of much less plasma or serum, natural solvent, and evaluation time in comparison with earlier CE-based assays and was efficiently utilized to watch ketamine, norketamine, 5,6-dehydronorketamine (DHNK), and 6-hydroxynorketamine (6HNK) stereoisomer ranges in plasma of a Beagle canine that obtained a bolus of racemic ketamine or S-ketamine after sevoflurane anesthesia. The info recommend that the formation of DHNK and 6HNK happen stereoselectively.
expressionpathology
expressionpathology

Discovery and microassay of a nitrite-dependent carbonic anhydrase exercise by stable-isotope dilution gasoline chromatography-mass spectrometry

The intrinsic exercise of carbonic anhydrase (CA) is the hydration of CO2 to carbonic acid and its dehydration to CO2. CA may additionally perform as esterase and phosphatase. Just lately, we demonstrated that renal CA is especially liable for the reabsorption of nitrite (NO2(-)) which is probably the most plentiful reservoir of the biologically extremely potent nitric oxide (NO). Via a stable-isotope dilution GC-MS technique, we found a novel CA exercise which strictly relies upon upon nitrite. We discovered that bovine erythrocytic CAII (beCAII) catalyses the incorporation of (18)O from H2 (18)O into nitrite at pH 7.4.
After derivatization with pentafluorobenzyl bromide, gasoline chromatographic separation and mass spectrometric evaluation, we detected ions at m/z 48 for singly (18)O-labelled nitrite ((16)O=N-(18)O(-)/(18)O=N-(16)O(-)) and at m/z 50 for doubly (18)O-labelled nitrite ((18)O=N-(18)O(-)) along with m/z 46 for unlabelled nitrite. Utilizing (15)N-labelled nitrite ((15)NO2 (-), m/z 47) as an inside commonplace and selected-ion monitoring of m/z 46, m/z 48, m/z 50 and m/z 47, we developed a GC-MS microassay for the quantitative dedication of the nitrite-dependent beCAII exercise.
The CA inhibitors acetazolamide and FC5 207A didn’t alter beCAII-catalysed formation of singly and doubly (18)O-labelled nitrite. Cysteine and the experimental CA inhibitor DIDS (a diisothiocyanate) elevated a number of fold the beCAII-catalysed formation of the (18)O-labelled nitrite species. Cysteine, acetazolamide, FC5 207A, and DIDS by themselves had no impact on the incorporation of (18)O from H2 (18)O into nitrite. We conclude that erythrocytic CA possesses a nitrite-dependent exercise which may solely be detected when nitrite is used because the substrate and the response is carried out in buffers of impartial pH values ready in H2 (18)O.
This novel CA exercise, i.e., the nitrous acid anhydrase exercise, represents a bioactivation of nitrite and will have each useful (by way of S-nitrosylation and subsequent NO launch) and presumably hostile (by way of C- and N-nitrosylation) results in residing organisms.

Characterization and enchancment of phenol-sulfuric acid microassay for glucose-based glycogen

OBJECTIVE
Phenol-sulfuric acid reagent is used to measure the focus of glyco-polymers and -conjugates. There are a number of uncertainties on glycogen measurement within the tissues. We aimed to enhance phenol-sulfuric reagent for microassay of glucose based-glycogen in small tube or microplate.
METHODS
The situation of the response was optimized and scaled down for each small tube and microplate software.
RESULTS
The colour depth was discovered to be a perform of all parts of the assay combination, that’s, the quantity of sugar and phenol along with the amount of whole water and acid. The absorbance elevated within the vary of 4-10 mg of phenol and reached the plateau between 10-16 mg per 1 mL of acid. The colour depth was a linear perform of whole water quantity (sugar-water- phenol). The sensitivity elevated eight occasions as whole water quantity was modified from 50 as much as 400 µL. The curve for acid quantity peaked at about 1 mL.
The optimum assay situation was decided to be 13 mg of phenol (200 µL 6.5%), 400-425 µL of whole water quantity (100 µL of sugar, 100 µL water) for 1 mL of acid. The preliminary spontaneous excessive temperature is crucial the response to proceed and any dealing with provides inconsistent outcomes and reduces the precision and sensitivity of the strategy. The values had been scaled down by an element of 0.5 for tube software and studying in cuvet or microplate and by 0.2 or 0.15 for microplate software.
CONCLUSIONS
The outcomes indicated that phenol-sulfuric acid reagent could possibly be scaled all the way down to 1.0, 0.5 and 0.20, 0.15 mL of sulfuric acid for microassay of glucose based-glycogen.

Recombinant Human Galectin-1 Protein
PROTP09382-1 50ug
EUR 317
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.

Anti-Galectin-3 Monoclonal Antibody
M00621-1 100ul
EUR 397
Description: Mouse Monoclonal Galectin-3 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

anti-Galectin 1
YF-PA12945 100 ug
EUR 403
Description: Rabbit polyclonal to Galectin 1

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349

Anti-Galectin 1 Antibody
A00470-2 100ug/vial
EUR 294

Anti-Galectin-1 antibody
STJ93201 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-1.

Anti-Galectin-1 antibody
STJ98716 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-1.

anti- Galectin-1 antibody
FNab03314 100µg
EUR 505.25
Description: Antibody raised against Galectin-1

anti- Galectin-1 antibody
FNab03315 100µg
EUR 548.75
Description: Antibody raised against Galectin-1

Anti-Galectin 1 (1A8)
YF-MA13984 100 ug
EUR 363
Description: Mouse monoclonal to Galectin 1

Anti-Galectin-1 antibody
PAab03314 100 ug
EUR 355

Anti-Galectin 1/LGALS1 Antibody
PA1422 100ug/vial
EUR 334

Anti-Galectin 1/LGALS1 Antibody
PB9240 100ug/vial
EUR 334

Anti-Galectin 1/Lgals1 Antibody
A00470 100ug/vial
EUR 334

Anti-Galectin 1 Biotinylated Antibody
A00470-Biotin 50ug/vial
EUR 294

Anti-galectin-1 (mouse) antibody
STJ72519 100 µg
EUR 359

anti-Galectin 1 (1E8-1B2)
LF-MA10174 100 ug
EUR 363
Description: Mouse monoclonal to Galectin 1

Anti-Galectin-13 (GAL13) / Placental Protein 13 (PP13) Monoclonal Antibody
M08143-1 100ug/vial
EUR 397
Description: Mouse Monoclonal Galectin-13 (GAL13) / Placental Protein 13 (PP13) Antibody. Validated in IHC and tested in Human.

anti-Galectin 3
YF-PA24079 50 ul
EUR 334
Description: Mouse polyclonal to Galectin 3

anti-galectin 9
YF-PA24081 50 ul
EUR 334
Description: Mouse polyclonal to galectin 9

anti-Galectin 3
YF-PA12946 50 ug
EUR 363
Description: Mouse polyclonal to Galectin 3

anti-Galectin 3
YF-PA12947 100 ul
EUR 403
Description: Rabbit polyclonal to Galectin 3

anti-Galectin 3
YF-PA12948 100 ug
EUR 403
Description: Rabbit polyclonal to Galectin 3

anti-Galectin 8
YF-PA12951 50 ul
EUR 363
Description: Mouse polyclonal to Galectin 8

anti-Galectin 13
YF-PA18585 50 ug
EUR 363
Description: Mouse polyclonal to Galectin 13

Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280

LGALS8 Human, Galectin-8 Human Recombinant Protein, His Tag
PROTO00214-1 Regular: 10ug
EUR 317
Description: LGALS8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37.9 kDa. The LGALS8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

LGALS3 Human, Galectin-3 Human Recombinant Protein, His Tag
PROTP17931-1 Regular: 25ug
EUR 317
Description: LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 270 amino acids (1-250 a.a.) and having a molecular mass of 28.3 kDa._x000D_ The LGALS3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. _x000D_

LGALS7 Human, Galectin-7 Human Recombinant Protein, His Tag
PROTP47929-1 Regular: 20ug
EUR 317
Description: Galectin-7 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids (1-136 a.a.) and having a molecular mass of 17.2kDa. ;The Galectin-7 is purified by proprietary chromatographic techniques.

Anti-human Galectin-3 antibody
STJ15100163 250 µg
EUR 336
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation

Human Galectin-3 (Gal-3) AssayMax ELISA Kit
EG3311-1 96 Well Plate
EUR 477

Human Galectin-4 (Gal-4) AssayMax ELISA Kit
EG3312-1 96 Well Plate
EUR 477

Human Galectin 1 Protein
20-abx066725
  • EUR 620.00
  • EUR 272.00
  • EUR 1859.00
  • EUR 732.00
  • EUR 453.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Recombinant Human Galectin-1
7-00427 10µg Ask for price

Recombinant Human Galectin-1
7-00428 50µg Ask for price

Recombinant Human Galectin-1
7-00429 1mg Ask for price

Human Galectin-1 (LGALS1)
1-CSB-EP012882HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Human Galectin-1 (LGALS1)
1-CSB-EP012882HUb1
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Galectin-1, human recombinant
4648-10
EUR 142

Galectin-1, human recombinant
4648-1000
EUR 1648

Galectin-1, human recombinant
4648-50
EUR 262

Anti-Galectin-9 Antibody
A03415 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Galectin-9 Antibody (LGALS9) detection.tested for WB in Human, Mouse, Rat.

Anti-Galectin-2 antibody
STJ93202 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-2.

Anti-Galectin-4 antibody
STJ93203 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-4.

Anti-Galectin-7 antibody
STJ93204 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-7.

Anti-Galectin-8 antibody
STJ93205 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-8.

Anti-Galectin-9 antibody
STJ93206 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-9.

Anti-Galectin-3 antibody
STJ96945 200 µl
EUR 197
Description: Galectin-3 is a protein encoded by the LGALS3 gene which is approximately 26,1 kDa. Galectin-3 is localised to the cytoplasm and nucleus. It is involved in NF-kappaB signalling, advanced glycosylation end product receptor signalling and cell adhesion. It is a galactose-specific lectin which binds IgE and may mediate the stimulation by CSPG4 of endothelial cells migration along with the alpha-3, beta-1 integrin. It is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. Galectin-3 is expressed mainly in the colonic epithelium and is also abundantly expressed in activated macrophages. Mutations in the LGALS3 gene may result in follicular adenoma and thyroid cancer. STJ96945 was developed from clone 6G2 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous galectin-3 proteins.

Anti-Galectin-3 antibody
STJ97549 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (6B8).

Anti-Galectin-3 antibody
STJ97550 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (8D7).

Anti-Galectin-3 antibody
STJ97551 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (5D9).

Anti-Galectin-3 antibody
STJ97601 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (2F9).

Anti-Galectin-3 antibody
STJ97604 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (16E6).

Anti-Galectin-3 antibody
STJ97605 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (1H4).

Anti-Galectin-3 antibody
STJ97715 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3.

Anti-Galectin 3 antibody
STJ73098 100 µg
EUR 359

Anti-Galectin-3 antibody
STJ16100369 1 mL
EUR 1047

Anti-Galectin-3 antibody
STJ16100782 100 µg
EUR 720

Anti-Galectin-3 antibody
STJ180106 0.1 ml
EUR 212

Anti-Galectin 3 antibody
STJ190057 200 µl
EUR 197
Description: Unconjugated Mouse monoclonal to Galectin 3 (AS1A24)

anti- Galectin 2 antibody
FNab03310 100µg
EUR 585
Description: Antibody raised against Galectin 2

anti- Galectin 4 antibody
FNab03311 100µg
EUR 505.25
Description: Antibody raised against Galectin 4

anti- Galectin 8 antibody
FNab03312 100µg
EUR 505.25
Description: Antibody raised against Galectin 8

anti- Galectin 9 antibody
FNab03313 100µg
EUR 505.25
Description: Antibody raised against Galectin 9

anti- Galectin-3 antibody
FNab03316 100µg
EUR 505.25
Description: Antibody raised against Galectin-3

anti- Galectin-3 antibody
FNab03317 100µg
EUR 548.75
Description: Antibody raised against Galectin-3

anti- Galectin-7 antibody
FNab03318 100µg
EUR 505.25
Description: Antibody raised against Galectin-7

anti-Galectin-3 (6G2)
LF-MA20336 100 ug
EUR 354
Description: Mouse monoclonal to Galectin-3

Anti-Galectin 8 (3E5)
YF-MA13986 100 ug
EUR 363
Description: Mouse monoclonal to Galectin 8

Anti-Galectin 2 antibody
PAab03310 100 ug
EUR 412

Anti-Galectin 4 antibody
PAab03311 100 ug
EUR 355

Anti-Galectin 8 antibody
PAab03312 100 ug
EUR 355

Anti-Galectin 9 antibody
PAab03313 100 ug
EUR 355

Anti-Galectin-3 antibody
PAab03316 100 ug
EUR 355

Anti-Galectin-7 antibody
PAab03318 100 ug
EUR 355

Anti-galectin-1 (mouse) (aa100-112) antibody
STJ72520 100 µg
EUR 359

Anti Human Galectin-3 Polyclonal Antibody
KH040
EUR 649
Description: The Anti Human Galectin-3 Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur.

Anti-Galectin-1 / Human Placental Lactogen (hPL) Monoclonal Antibody
M00470 100ug/vial
EUR 397
Description: Mouse Monoclonal Galectin-1 / Human Placental Lactogen (hPL) Antibody. Validated in IHC and tested in Human.

Galectin-1 Protein
20-abx261634
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Galectin-1 Protein
20-abx263505
  • EUR 230.00
  • EUR 1790.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug

Galectin-1 (LGAS1)
PR27143 50 ug
EUR 318

Galectin 1 Antibody
49673-100ul 100ul
EUR 333

Galectin 1 Antibody
49673-50ul 50ul
EUR 239

rHu Galectin-1
AK8281-0010 10µg Ask for price

rHu Galectin-1
AK8281-0050 50µg Ask for price

rHu Galectin-1
AK8281-0100 100µg Ask for price

rHu Galectin-1
AK8281-1000 1mg Ask for price

Galectin-1 Antibody
3596-100
EUR 316

Galectin-1 Antibody
3596-30T
EUR 146

Galectin 1 antibody
70R-GR002 100 ug
EUR 476
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody

Galectin 1 protein
30R-1385 100 ug
EUR 224
Description: Purified recombinant Human Galectin 1 protein

Galectin 1 protein
30R-2371 50 ug
EUR 353
Description: Purified recombinant Human Galectin 1 protein

Galectin 1 protein
30R-AG003 10 ug
EUR 133
Description: Purified recombinant Human Galectin 1 protein

Galectin 1 antibody
70R-11939 100 ug
EUR 403
Description: Rabbit polyclonal Galectin 1 antibody

Galectin 1 antibody
70R-13963 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody