spherical RNAs symbolize a small proportion of full RNA RNase R

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• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are generally required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement additional enzyme partway (e.g. 0.5 μl after 1 hour) by way of the method. The higher temperature is particularly useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this may end in non-enzymatic RNA degradation.

• RNase R shows low train on tRNA, rRNA and totally different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use additional RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Keep in mind that spherical RNAs symbolize a small proportion of full RNA (generally 0.1%-0.01%), because of this reality RNase R treatment will most actually finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that purpose, a starting amount of not lower than 10 µg of full RNA is useful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be useful since extreme heat can lead to RNA hurt. Phenol-chloroform precipitation could be utilized instead. For NGS, robust half reversible immobilization (SPRI) bead cleanup is useful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

Biovision Rnase-R
Biovision Rnase-R

DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by typical splicing, along with circRNAs which come up by way of back-splicing.

APPLICATIONS:
• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying varied splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Stay away from repeated freeze-thaw cycles of all components to retain most effectivity. All components
are regular for one 12 months from the date of transport when saved and handled appropriately.

 

Cat # +DimensionM1228-500
Dimension500 U
HighlightsRNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions-20°C
Supply ConditionsGel Pack
USAGEFor Evaluation Use Solely! Not For Use in Individuals.

 

 

RNAse

E16FXP0211R-400 400 μl
EUR 20
Description: Available in various conjugation types.

RNAse

E16FXP0211R-800 800 μl
EUR 26.67
Description: Available in various conjugation types.

RNase A

abx098139-1ml 1 ml
EUR 260.4

PI / RNAse

abx290029-200tests 200 tests
EUR 326.4

PI/RNAse

PI/RNASE 200 test
EUR 208.2

RNase A

M1227-25 each
EUR 294

RNase A

RN103-001 300mg
EUR 3138

RNase A

RB0473 500mg
EUR 219.73

RNase A

IB47163 50ul
EUR 20.72
Description: (FOR HI-SPEED PLASMID) [FR}

RNase H

RK20535 250U
EUR 88

RNase FREE, 250ml Bottle Removes RNase & DNAse

10-172 1 Bottle/Unit
EUR 269

Rnase (90–105)

5-01885 4 x 1mg Ask for price

Rnase A, 250mg

R9001-250mg each
EUR 337.2

RNase Inhibitor

EQ010 1000U
EUR 33.5

RNase FREE, 1 Liter Bottle Removes RNase & DNAse

10-173 1 Bottle/Unit
EUR 337

RNase FREE, 4 Liter Bottle Removes RNase & DNAse

10-456 1 Bottle/Unit
EUR 403

RNase FREE, 475ml Spray Bottle Removes RNase & DNAse

10-228 1 Spray Bottle/Unit
EUR 300

Rnase A, 1g

R9001-1g each
EUR 986.4

Rnase A, 1g

R9001 1g
EUR 667.9

RNase A (MBG)

409-050 50 mg
EUR 87.6

RNase A (MBG)

409-250 250 mg
EUR 231.6

RNase L Antibody / Rnasel

RQ6435 100 ug
EUR 356.15
Description: RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.

RNase III, E.coli

M1226-100 each
EUR 268.8

RNase L Peptide

46-735P 0.1 mg
EUR 405.6
Description: ABCE1 / RNase L Peptide

RNase T2 Antibody

abx237324-100ug 100 ug
EUR 610.8

RNase 1 Antibody

20-abx141499
  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

RNase 13 Antibody

20-abx141582
  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

RNAse H2A Peptide

4979P 0.05 mg
EUR 197.7
Description: (IN) RNAse H2A peptide

RNase A Solution

G118 1.0 ml; 10 mg/ml
EUR 122.4