AccuCount Particles

Contains particles with a known number of particles per mL

AccuCount Fluorescent Particles

• Easy to use and cost effective
• Available in several sizes to accommodate the target cell size to be counted
• Provided as blank, fluorescent, Rainbow, or Ultra Rainbow particles that can be used in multiple fluorescent channels of flow cytometers.

The SPHEROTM AccuCount Particles are designed to be used as reference particles with known number of particles per mL for counting the absolute cell number by flow cytometry. The SPHEROTM AccuCount Particles are very easy to use and are cost effective. The AccuCount Fluorescent Particles are fluorescent in FITC, PE and PE-Cy5 channel.

AccuCount Blank Particles | ACBP-50-10

Both AccuCount Fluorescent and AccuCount Blank (nonfluorescent) Particles are available in various particle sizes to accommodate the size of the cells to be counted. In addition, Spherotechalso manufactures the AccuCount Rainbow Fluorescent Particles and AccuCount Ultra Rainbow Fluorescent Particles for detection in more fluorescent channels.

spherotech accucount blank
spherotech accucount blank flow cytometry

Product Description

Category Micro/Nanoparticles
Supplier Spherotech
Regulatory Status RUO
Concentration 1E6/mL
Storage Conditions 2-8° C
Product Line Research
Tech. Data Sheet View datasheet
Link To Supplier http://www.spherotech.com
Shipping Details Cold
Material Safety Data Sheet Download PDF

 

Introduction
Assays for cell counting using flow cytometry and calibrated fluorescent particles are rapid and accurate. The single platform method that enumerates T-cells by counting the identifier cells in either a precise known cell volume or an internal ‘spike’ of a known number of calibrated fluorescent particles by flow cytometry is simple and efficient (1). These assays allow the counting of T-cells during anti-T-cell globulin treatment of cardiac, lung, and renal transplant patients (2). In addition, laboratories can determine the absolute count of CD4 and CD8 Tcells to estimate HIV disease progression with the single platform method (3). Calibrated fluorescent particles and
flow cytometry are also used to count platelets in a wide range of murine models of platelet disorders (4). It is also possible to count other various cell types with flow cytometry and calibrated fluorescent particles.

The accurate quantitation of cells in blood is crucial for precise determination of treatment procedures during clinical care. Flow cytometry and calibrated fluorescent particles have improved cell counting methods over predicated methods. In the past, multi-platform methods were used to quantitate T-cells. The conventional multi-platform method uses cell marker percentages from the flow cytometer and white blood cell count, percent lymphocytes, and absolute lymphocyte count from a hematology analyzer to enumerate T-cells in blood. Substantial variations in data have resulted between different laboratories using multi-platform methods.

sphero accucount

These variations are due toan error created in each independent measurement which multiplies at consecutive steps during calculations. It is assured that the single-platform CD4 and CD8 T-cell determination technique is an acceptable alternative to the multi-platform method. Single-platform assays using a known number of reference particles resulted in significant and substantial improvements in laboratory-to-laboratory and within-laboratory precision over multiplatform methods for T-cell enumeration (1).

 

AccuCount Fluorescent Particles

ACFP-70-10 10 mL
EUR 352.8
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particles

ACFP-70-5 5 mL
EUR 230.4
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particles

ACFP2.5-30056-1 1 mL
EUR 157.2
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particles

ACFP20-10056-1 1 mL
EUR 128.4
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particles

ACFP5-15056-1 1 mL
EUR 140.4
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particles

ACFP5-20056-1 1 mL
EUR 128.4
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particles

ACFP50-7056-1 1 mL
EUR 133.2
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Fluorescent Particle Kit

ACFP-50-4K 4X1mL
EUR 620.4
Description: Flow Cytometry Particles For Absolute Cell Counting

AccuCount Rainbow Fluorescent Particles

ACRFL-100-3 3 mL
EUR 254.4
Description: Flow Cytometry Particles For Fine Tuning Cell Sorters

AccuCount Rainbow Fluorescent Particles

ACRFP-100-3 3 mL
EUR 254.4
Description: Flow Cytometry Particles For Fine Tuning Cell Sorters

AccuCount Ultra Rainbow Fluorescent Particles

ACURFP-38-15 15 mL
EUR 535.2
Description: Flow Cytometry Particles For Fine Tuning Cell Sorters

AccuCount Ultra Rainbow Fluorescent Particles

ACURFP-38-5 5 mL
EUR 249.6
Description: Flow Cytometry Particles For Fine Tuning Cell Sorters

AccuCount Ultra Rainbow Fluorescent Particles

ACURFP-50-10 10 mL
EUR 364.8
Description: Flow Cytometry Particles For Fine Tuning Cell Sorters

AccuClear dsDNA Standard, 25 ng/uL

31029C 1mL
EUR 111.6
Description: Minimum order quantity: 1 unit of 1mL

Accuris AccuCheck Pipette Calibration System (W3002A-120 Balance with W3110 Anti Evaporation Trap)

W3002A-120ACS 1 each
EUR 4115.6

AccuClear Ultra High Sensitivity dsDNA Quantitation Solution (1000 assays)

31027 1kit
EUR 298.8
Description: Minimum order quantity: 1 unit of 1kit

AccuClear Ultra High Sensitivity dsDNA Quantitation Solution, Trial Size (200 assays)

31027-T 1kit
EUR 134.4
Description: Minimum order quantity: 1 unit of 1kit

AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards (1000 assays)

31028 1kit
EUR 434.4
Description: Minimum order quantity: 1 unit of 1kit

AccuClear Ultra High Sensitivity dsDNA Quantitation Assay kit with 1 DNA Standard (2000 assays)

31029 1kit
EUR 616.8
Description: Minimum order quantity: 1 unit of 1kit

AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size (200 assays)

31028-T 1kit
EUR 148.8
Description: Minimum order quantity: 1 unit of 1kit

1L AccuGel 19:1 (30%)

NAT1178 1L
EUR 156

1L AccuGel 19:1 (40%)

NAT1182 1L
EUR 154.8

1L AccuGel 29:1 (30%)

NAT1186 1L
EUR 156

1L AccuGel 29:1 (40%)

NAT1190 1L
EUR 158.4

Accuris

W1105-20 1 PC
EUR 91.92

Accuris

W1105-500 1 PC
EUR 118.9

Accuris

W1105-9-1000 1 PC
EUR 223.3

Accudenz 50g

AN7050 50 gm
EUR 244.8
Description: Cell Separation Media

Accudenz 500g

AN7050/BLK 500 gm
EUR 1340.4
Description: Cell Separation Media

 

Sphero datasheets can be found here.