Recombinant proteinase K is extremely efficient on native proteins

Calcium is important for the thermostability of proteinase K and the pH value is critical for high solubility

 

The use of cryoprotectant glycerol to a final concentration of 50% in the stock solution is to prevent freeze / thaw damage. Proteinase K retained 99% of the activity after 12 freeze / thaw cycles in the stock solution. Avoid repeated freezing and thawing to avoid protein precipitation if glycerol is not added.

Proteinase K solution remains active in a wide pH range (4.0-12.5, often used in the 7.5-9.0 range) and in a wide temperature range of 25-75 ° C. The solution remains sterile and stable for at least 12 months at 4 ° C.

Recombinant proteinase K is a nonspecific serine protease that is useful for general protein digestion.

Proteinase K is a highly reactive serine protease that shows the ability to digest native proteins, thus inactivating enzymes such as DNase and RNase without resorting to a denaturation process. It is the most potent proteinase among all the proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic, or hydrophobic amino acids.

Recombinant proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature compared to native proteinase K.

Recombinant proteinase K is expressed from the yeast Pichia pastoris with a cloned gene encoding the endolytic protease of Engyodontium album (Tritirachium album).

Proteinase K Recombinant Protein
Proteinase K Recombinant Protein

Applications

• Recombinant proteinase K is extremely efficient on native proteins and therefore can be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.

• It is used for the analysis of membrane structures by modifying proteins and glycoproteins on the cell surface.

• Particularly suitable for isolating nucleic acids for amplification reactions.

• Promotes cell lysis by activating a bacterial autolytic factor.

• Used to remove cell debris during colony lift preparation and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

Reconstitution Recommendations

For the preparation of the stock solution, we suggest reconstituting the lyophilized powder in a buffer (20 mM Tris, 1 mM CaCl2, 2% glycerol, pH 7.4).

 

 

DNA / RNA Damage Antibody

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DNA / RNA Damage Antibody

20-abx444884
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  • Ask for price
  • 100 ug
  • 25 ug

DNA / RNA Damage Antibody

abx444884-100l 100 µl
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DNA / RNA Damage Antibody (PE)

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SMC-155C 0.025mg
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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

DNA/RNA Damage Antibody, Clone 15A3

SMC-155D 0.1mg
EUR 306
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

DNA/RNA Damage Antibody, Clone 15A3

SMC-155S 0.012mg
EUR 44
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

OASE00080-25UG - DNA/RNA Damage Antibody

OASE00080-25UG 25ug
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OASE00081-100UG - DNA/RNA Damage Antibody

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Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC.

DNA/RNA Damage Antibody, Clone 15A3: HRP

SMC-155D-HRP 0.1mg
EUR 345
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with HRP.

DNA/RNA Damage Antibody, Clone 15A3: RPE

SMC-155D-RPE 0.1mg
EUR 355
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with RPE.

DNA/RNA Damage Antibody, Clone 15A3: FITC

SMC-155D-FITC 0.1mg
EUR 350
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with FITC.

DNA/RNA Damage Antibody, Clone 15A3: PerCP

SMC-155D-PCP 0.1mg
EUR 358
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with PerCP.

DNA/RNA Damage Antibody, Clone 15A3: Biotin

SMC-155D-BI 0.1mg
EUR 354
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Biotin.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A565 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 565.

Monoclonal antibody for DNA/RNA Damage

SMC-155D-A633 0.1mg
EUR 453.6
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 633.