chromatin concentration and DNA fragmentation

Storage

JC-1 Assay Buffer (10 ×) [E-CK-A301B] should be store at 2~8°C, other reagent should be stored at -20°C.

JC-1 Reagent [E-CK-A301A] should be stored in dark. Avoid freeze / thaw cycles.

Cautions

  1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
  2. JC-1 Reagent may coagulate or precipitate at lower temperatures. Please heat at 20~25°C water bath until it is completely dissolved.
  3. IF the cells sensitive to pH changes, please use fetal bovine serum to replace JC-1 Assay Buffer for incubation or prolong observation time.
  4. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
  5. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Introduction

Elabscience Mitochondrial Membrane Potential Assay Kit (with JC-1) is developed to identify apoptotic with JC-1 reagent by changing of mitochondrial membrane potential which occur in early apoptotis.

This kit provides Carbonyl cyanide m-chlorophenylhydrazone (CCCP)[E-CK-A301C] to induce the decrease of mitochondrial membrane potential as a positive control reagent.

Detection principle

The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic characteristics (chromatin concentration and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible.

In normal cells, the mitochondrial membrane potential is high, JC-1 gathers in the matrix of mitochondria to form a polymer, which yields a red to orange colored emission (590±17.5 nm). In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t gather and it is a monomer which yields green fluorescence with emission of 530±15 nm.

Components

Cat. Products 20 Assays 50 Assays 100 Assays Storage
E-CK-A301A  JC-1 Reagent 20 μL 50 μL 100 μL -20°C
E-CK-A301B  JC- 1 Assay Buffer (10 ×) 4 mL 10 mL 10 mL× 2 2~8°C
E-CK-A301C  CCCP(10 mM) 40 μL 40 μL 40 μL -20°C
Manual  One Copy
bitopt tmrm assay
bitopt tmrm assay

Reagents not included

PBS, DI water.

Instructions

1×JC-1 Assay Buffer preparation

JC- 1 Assay Buffer (10 ×)[E-CK-A301B] is concentrated, diluted with DI water to 1 × working solution before use. Store at 2~8°C.

For example: take 100 μL JC- 1 Assay Buffer (10 ×), add to 900 μL DI water, the mixture is 1× JC- 1 Assay Buffer.

JC-1 Staining Buffer preparation

  1. Take100 μL JC- 1 Assay Buffer (10 ×), add to 900 μL DI water, mix and heat to 37°C.
  2. Add 2 μL JC-1 Reagent [E-CK-A301A] to the mixture.Mix until JC-1 Reagent fully dissolved. The mixture is JC-1 Staining Buffer.

Tip: JC-1 has a low solubility in water, it can be heated at 37°C to promote dissolution.

Positive Control preparation

  1.  CCCP(10 mM)[ E-CK-301C] dilute at 1:1000 to cell culture medium, and the CCCP is at 10 μM. Add the cell culture medium to cell and incubate for 20 min.
  2.  Follow the experiment procedure to detect themitochondrial membrane potential.

Tips: For most cells, the mitochondrial membrane potential would be completely lost after 20 min of CCCP treatment at 10 μM and JC-1 stained cells showed green fluorescence, while normal cells showed red fluorescence after JC-1 staining.

        For specific cells, the concentration and the incubation time of CCCP may be different, please refer to the relevant literature to determine.

 

JC-1 (Mitochondrial membrane-potential dependent fluorescent dye)
SIH-562-5MG 5 mg
EUR 339.6
Description: The substance JC-1 is a mitochondrial membrane-potential dependent fluorescent dye. It is synthetically produced and has a purity of >98%. The pure substance is red powder which is May be dissolved in water (50 mg/ml); DMSO (15 mg/ml).

Screen Quest™ Membrane Potential Assay Kit *Orange Fluorescence*
35999 1 plate
EUR 262.8

Screen Quest™ Membrane Potential Assay Kit *Orange Fluorescence*
36000 10 plates
EUR 576

Screen Quest™ Membrane Potential Assay Kit *Orange Fluorescence*
36001 100 plates
EUR 4183.2

Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence*
36004 1 plate
EUR 262.8

Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence*
36005 10 plates
EUR 576

Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence*
36006 100 plates
EUR 4183.2

JC-1 Mitochondrial Membrane Potential Detection Kit (25 assays)
30001-T 1kit
EUR 152.4
Description: Minimum order quantity: 1 unit of 1kit

MycoLight™ Ratiometric Bacterial Membrane Potential Kit *Red/Green Fluorescence*
22401 200 Tests
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Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*
22802 100 Tests
EUR 262.8

Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Reader*
22803 500 Tests
EUR 262.8

Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*
22800 500 Tests
EUR 367.2

Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
22804 100 Tests
EUR 262.8

Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader*
22805 500 Tests
EUR 262.8

Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
22806 100 tests
EUR 262.8

Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Red Fluorescence Optimized for Microplate Reader*
22807 500 tests
EUR 262.8

Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*
22801 100 Tests
EUR 367.2

Dialysis Membrane
2392344 8unit
EUR 331.2

Membrane Remover
MVSMR100 Complete
EUR 3183.6

RO Membrane
WAT2178 EACH
EUR 850.44

Spoligotyping Membrane
IM9702 each
EUR 1536
Description:

A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research. Spoligotyping is a PCR-based Method to Simultaneously Detect and Type Mycobacterium Tuberculosis Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type M. tuberculosis complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.). The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus, Spoligotyping is a preferred method to type M. bovis strains, which usually contain only one or two IS6110 copies. Note, that Mycobacterium bovis can be recognized by the absence of reactivity with spacers 39-43.

TMB Membrane Substrate
42-TB07 100 ml
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Description: High sensitivity HRP membrane substrate (containing TMB)

Brain Membrane Lysate
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Description: Monkey (Cynomolgus) brain tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) brain tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated brain tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Brain Membrane Lysate
21-241 0.1 mg
EUR 619.8
Description: Monkey (Rhesus) brain tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) brain tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated brain tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Heart Membrane Lysate
21-243 0.1 mg
EUR 619.8
Description: Monkey (Rhesus) heart tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) heart tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated heart tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Brain Membrane Lysate
21-358 0.1 mg
EUR 619.8
Description: Mouse brain tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The mouse brain tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated brain tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Brain Membrane Lysate
21-440 0.1 mg
EUR 619.8
Description: Rat brain tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat brain tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated brain tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Zika Membrane Antibody
8667-002mg 0.02 mg
EUR 206.18
Description: Zika virus (ZIKV) is a member of the virus family Flaviviridae. It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Zika virus is related to the dengue, yellow fever, Japanese encephalitis, and West Nile viruses. Zika often causes no or only mild symptoms, similar to a very mild form of dengue fever. Zika can also spread from a pregnant woman to her fetus. This can result in microcephaly, severe brain malformations, and other birth defects. This antibody is specific to the Membrane protein.

Zika Membrane Antibody
8667-01mg 0.1 mg
EUR 523.7
Description: Zika virus (ZIKV) is a member of the virus family Flaviviridae. It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Zika virus is related to the dengue, yellow fever, Japanese encephalitis, and West Nile viruses. Zika often causes no or only mild symptoms, similar to a very mild form of dengue fever. Zika can also spread from a pregnant woman to her fetus. This can result in microcephaly, severe brain malformations, and other birth defects. This antibody is specific to the Membrane protein.

Zika Membrane Peptide
8667P 0.05 mg
EUR 197.7
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Nitrocellulose Membrane Roll
B500 3m x 30cm Roll
EUR 326.4

Nitrocellulose Membrane Precut
B501 Pack of 30, 6cm x 8.5cm
EUR 148.8