chromatin concentration and DNA fragmentation

Storage

JC-1 Assay Buffer (10 ×) [E-CK-A301B] should be store at 2~8°C, other reagent should be stored at -20°C.

JC-1 Reagent [E-CK-A301A] should be stored in dark. Avoid freeze / thaw cycles.

Cautions

  1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
  2. JC-1 Reagent may coagulate or precipitate at lower temperatures. Please heat at 20~25°C water bath until it is completely dissolved.
  3. IF the cells sensitive to pH changes, please use fetal bovine serum to replace JC-1 Assay Buffer for incubation or prolong observation time.
  4. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
  5. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Introduction

Elabscience Mitochondrial Membrane Potential Assay Kit (with JC-1) is developed to identify apoptotic with JC-1 reagent by changing of mitochondrial membrane potential which occur in early apoptotis.

This kit provides Carbonyl cyanide m-chlorophenylhydrazone (CCCP)[E-CK-A301C] to induce the decrease of mitochondrial membrane potential as a positive control reagent.

Detection principle

The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic characteristics (chromatin concentration and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible.

In normal cells, the mitochondrial membrane potential is high, JC-1 gathers in the matrix of mitochondria to form a polymer, which yields a red to orange colored emission (590±17.5 nm). In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t gather and it is a monomer which yields green fluorescence with emission of 530±15 nm.

Components

Cat.Products20 Assays50 Assays100 AssaysStorage
E-CK-A301A JC-1 Reagent20 μL50 μL100 μL-20°C
E-CK-A301B JC- 1 Assay Buffer (10 ×)4 mL10 mL10 mL× 22~8°C
E-CK-A301C CCCP(10 mM)40 μL40 μL40 μL-20°C
Manual One Copy
bitopt tmrm assay
bitopt tmrm assay

Reagents not included

PBS, DI water.

Instructions

1×JC-1 Assay Buffer preparation

JC- 1 Assay Buffer (10 ×)[E-CK-A301B] is concentrated, diluted with DI water to 1 × working solution before use. Store at 2~8°C.

For example: take 100 μL JC- 1 Assay Buffer (10 ×), add to 900 μL DI water, the mixture is 1× JC- 1 Assay Buffer.

JC-1 Staining Buffer preparation

  1. Take100 μL JC- 1 Assay Buffer (10 ×), add to 900 μL DI water, mix and heat to 37°C.
  2. Add 2 μL JC-1 Reagent [E-CK-A301A] to the mixture.Mix until JC-1 Reagent fully dissolved. The mixture is JC-1 Staining Buffer.

Tip: JC-1 has a low solubility in water, it can be heated at 37°C to promote dissolution.

Positive Control preparation

  1.  CCCP(10 mM)[ E-CK-301C] dilute at 1:1000 to cell culture medium, and the CCCP is at 10 μM. Add the cell culture medium to cell and incubate for 20 min.
  2.  Follow the experiment procedure to detect themitochondrial membrane potential.

Tips: For most cells, the mitochondrial membrane potential would be completely lost after 20 min of CCCP treatment at 10 μM and JC-1 stained cells showed green fluorescence, while normal cells showed red fluorescence after JC-1 staining.

        For specific cells, the concentration and the incubation time of CCCP may be different, please refer to the relevant literature to determine.

 

Elite Mitochondrial Membrane Potential Kit

MBS430187-960Assays 960Assays
EUR 500

DiO/DPA Membrane Potential Detection Kit

30037-1 KT
EUR 256

Mitochondrial membrane potential detection kit

BC157-50T 50T
EUR 200

Elite Membrane Potential Dye Kit (Fluorescent)

MBS433552-10Plates 10Plates
EUR 725

Elite Membrane Potential Dye Kit (Fluorescent)

MBS433552-5x10Plates 5x10Plates
EUR 3335

JC1 Mitochondrial Membrane Potential Assay Kit

abx090685-100tests 100 tests
EUR 774

JC1 Mitochondrial Membrane Potential Assay Kit

abx090685-100l 100 µl
EUR 500

JC1 Mitochondrial Membrane Potential Assay Kit

abx090685-1ml 1 ml Ask for price

JC1 Mitochondrial Membrane Potential Assay Kit

abx090685-200l 200 µl Ask for price

TMRE mitochondrial Membrane Potential Assay Kit

K2233-100 100T
EUR 120.8
Description: Cell Biology|Cell Apoptosis

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11298)

OKSA11298 500 Tests
EUR 1147.2
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

MITOCHONDRIAL MEMBRANE POTENTIAL KIT Kits (OKSA11300)

OKSA11300 500 Tests
EUR 1147.2
Description: Description of target: MITOCHONDRIAL MEMBRANE POTENTIAL KIT ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

JC-1 Mitochondrial membrane potential assay kit

K2002-100 100T
EUR 90.4
Description: Cell Biology|Cell Apoptosis

Mitochondrial Membrane Potential Assay Kit (JC-1)

KTA4001-100T 100 T
EUR 149
Description: Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1) provides a simple method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential.

Mitochondrial Membrane Potential Assay Kit (JC-1)

KTA4001-20T 20 T
EUR 49
Description: Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1) provides a simple method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential.

Mitochondrial Membrane Potential Assay Kit (JC-1)

KTA4001-500T 500 T
EUR 449
Description: Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1) provides a simple method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential.

Mitochondrial Membrane Potential Assay Kit (JC-1)

MBS9711517-100Tests 100Tests
EUR 230

Mitochondrial Membrane Potential Assay Kit (JC-1)

MBS9711517-20Tests 20Tests
EUR 170

Mitochondrial Membrane Potential Assay Kit (JC-1)

MBS9711517-500Tests 500Tests
EUR 405

Mitochondrial Membrane Potential Assay Kit (JC-1)

MBS9711517-5x500Tests 5x500Tests
EUR 1815

Rhodamine 123 mitochondrial Membrane Potential Assay Kit

K2232-100 100T
EUR 53.6
Description: Cell Biology|Cell Apoptosis

Mitochondrial Membrane Potential Assay Kit (with JC-1)

E-CK-A301-100Assays 100 Assays
EUR 369

Mitochondrial Membrane Potential Assay Kit (with JC-1)

E-CK-A301-20Assays 20 Assays
EUR 108

Mitochondrial Membrane Potential Assay Kit (with JC-1)

E-CK-A301-50Assays 50 Assays
EUR 216

Mitochondrial membrane potential assay kit with JC-1

EBA1410 10 assays
EUR 80

Mitochondrial membrane potential assay kit with JC-1

EBA14100 100 assays
EUR 440

Mitochondrial membrane potential assay kit with JC-1

EBA1420 20 assays
EUR 120

Mitochondrial membrane potential assay kit with JC-1

EBA1450 50 assays
EUR 240

Mitochondrial Membrane Potential Assay Kit (with JC-1)

E-CK-A301-each each Ask for price
Description: Apoptosis/JC-1

Mitochondrial Membrane Potential Assay Kit with JC-1

MBS8579980-100Tests 100Tests
EUR 215

Mitochondrial Membrane Potential Assay Kit with JC-1

MBS8579980-50Tests 50Tests
EUR 180

Mitochondrial Membrane Potential Assay Kit with JC-1

MBS8579980-5x100Tests 5x100Tests
EUR 925