Ago2 Antibody, Brachyury Antibody, Brn3A Antibody, Cd45Ro Antibody, Cdk5 Antibody, Erg Antibody, Foxa2 Antibody, Galr1 Antibody, Goat, Guinea, Human, Influenza, Insect, Jmjd3 Antibody, Kangaroo, Killifish, Lkb1 Antibody, Parp1 Antibody, Pig, Raccoon, Reindeer, Reptile, Tbp Antibody, Zeb2 Antibody
A microassay for urinary phenol using capillary gas chromatography and optimised enzymic hydrolysis
An improved capillary gasoline chromatography technique is described for the assay of urinary phenol for the evaluation of benzene publicity. Enzymatic hydrolysis with an extract of Helix pomatia is used to liberate phenol from its glucuronide and sulphate conjugates in urine and has the benefit in that full hydrolysis happens and clear chromatograms are produced.
Moreover, an inner customary (2,4-xylenol) has been integrated within the technique enabling fast and single step extraction of phenol. The microassay can be utilized to measure phenol concentrations as little as 1 mg/l in urine thus making it an appropriate technique for monitoring benzene publicity.
A microdetermination of propranolol enantiomers and of their glucuronide and sulphate conjugates in human plasma and urine by reversed-phase HPLC after chiral derivatization is described. After extraction from 100 microliters of plasma or urine with racemic 4-methylpropranolol as inner customary (I.S.), the enantiomers are derivatized with R(+)-phenylethylisocyanate as chiral derivatization reagent. Chromatography is carried out on Novapak C18 column with fluorescence detection. Glucuronide and sulphate conjugates are cleaved previous to extraction by incubating, respectively, the samples with glucuronidase-arylsulphatase and saccharic acid 1-Four lactone as particular glucuronidase inhibitor.
The retention instances of propranolol and I.S. enantiomer derivatives are brief (tR = 5.5-6.2 min and eight.8-10.1 min, respectively). The diastereomeric derivatives are very secure and present good peak symmetry and resolutions (RS = 2 and a pair of.2). The usage of 4-methylpropranolol as I.S. improves considerably relative customary deviations (RSD: 1.7-5.1). Sensitivity is about 1 ng ml-1 per enantiomer. The tactic is utilized to pharmacokinetic research of racemic propranolol in human plasma and urine. S-propranolol and its conjugates present larger concentrations than R-propranolol and its conjugates in plasma and urine.
Microassay for oligosaccharyltransferase: separation of response elements by partitioning in detergent answer adopted by ultrafiltration
Oligosaccharyltransferase catalyzes the switch of oligosaccharide from a lipid dolichol to asparagine acceptor websites on nascent polypeptides. Now we have developed an assay for this enzyme which relies on the particular distribution of the substrate and product of the response in detergent answer. GlcNAc-[3H]GlcNAc-PP-Dol was synthesized to be used as a carbohydrate donor. Benzoyl-Asn-Leu-Thr-amide, a commercially accessible peptide, was used because the oligosaccharyltransferase glycan acceptor substrate.
Within the presence of Triton X-100, GlcNAc-[3H]GlcNAc-PP-Dol partitions into detergent micelles whereas glycosylated acceptor peptide partitions into the intermicellar aqueous compartment. Separation of GlcNAc-[3H]GlcNAc-PP-Dol and glycopeptide was achieved by ultrafiltration. With this technique oligosaccharyltransferase exercise in microsomal preparations may very well be measured with as little as 1 microgram of protein.
A fast microassay for dichloroacetate in serum by gel-permeation chromatography.
Now we have developed a novel, fast microassay for dichloroacetate within the serum. The serum pattern is immediately injected right into a gel-permeation high-performance liquid chromatography equipment. The height of dichloroacetate seems after an enormous protein peak. The tactic requires a really small quantity of serum (10 microliters), and the evaluation time is brief (20 min). Utilizing this micro technique, we measured the serum concentrations of dichloroacetate in wholesome grownup volunteers and paediatric sufferers with congenital lactic acidosis.
Though the impact of dichloroacetate on the neurological manifestations of congenital lactic acidosis has not been proved to be useful, the potential usefulness of dichloroacetate in refractory lactic acidosis in cardiac and respiratory failure has been acknowledged, and human in addition to animal research have been undertaken in lots of laboratories. To forestall doable negative effects of dichloroacetate, it has been beneficial that the minimal efficient dose be used. Our microassay technique is helpful for each human and animal experiments, even after administration of minimal doses.
A fluorescence microassay for the quantitation of integrin-mediated adhesion of neutrophil
The avidity of leukocyte integrin CR3 (Mac-1, CD11b/CD18, alpha m beta 2) on neutrophils (PMN) could also be quickly modulated by a number of agonists. We describe a technique for figuring out the avidity of those receptors by measuring the adhesion of PMN to fibrinogen-coated surfaces. Cells are loaded with a succinimidyl ester of carboxyfluorescein diacetate, which is deacetylated by intracellular esterases yielding a extremely fluorescent carboxyfluorescein that continues to be trapped inside the PMN.
The variety of cells adhering to fibrinogen-coated wells of Terasaki microplates is quantitated with a fluorescence plate reader. Stimulation of PMN with quite a few agonists, together with PMA, fNLLP, Ca-ionophore A23187, interleukin-8, tumor necrosis issue and lipopolysaccharide strongly elevated adhesion to fibrinogen, which was CD11b/CD18 dependent. The extent of cell adhesion trusted stimulus focus and incubation time. This assay requires little time, makes use of small numbers of cells and doesn’t require hazardous reagents.
A microassay for measuring cytochrome P450IA1 and P450IIB1 actions in intact human and rat hepatocytes cultured on 96-well plates
This examine offers with the event of a delicate and easy microassay for measuring cytochrome P450IA1 and P450IIB1 actions in intact human and rat hepatocytes. The tactic relies on the metabolism by cells cultured in microwells of acceptable substrates at noncytotoxic concentrations (Eight microM 7-ethoxyresorufin and 15 microM 7-pentoxyresorufin). After incubation of the probes with the cells, the dealkylated resorufin shaped and launched into tradition medium was quantified.
To make sure the hydrolysis of the resorufin conjugates ultimately shaped, tradition supernatants had been incubated within the microwells with beta-glucuronidase and arylsulfatase. The fluorescence was then learn utilizing a microplate fluorescence reader. A excessive correlation between the monooxygenase exercise measured by this process and that measured by typical procedures within the microsomal fraction of the identical cells was discovered.
The main benefits of this technique are: (1) the small variety of cells required; (2) a drastic discount in assay time; (3) that the assay is carried out in intact cells; and (4) the potential of performing repeated assays with the identical cell monolayer over a interval of a number of days since no damage to cells is detectable in the course of the assay. This technique proved to be very handy for learning cytochrome P450 induction by xenobiotics in main cultures of human hepatocytes.
 Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue II |
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| T8334608 | Biochain | 5 slides | EUR 242 |
 Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue III |
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| T8334423 | Biochain | 5 slides | EUR 242 |
 High density tissue microarray of Hodgkin's Disease |
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| LM208 | TissueArray | each | EUR 546 |
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Description: High density tissue microarray of Hodgkin's Disease, Non-Hodgkin's lymphoma and normal lymph node tissues, 69 cases/208 cores |
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 Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue I |
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| T8434448 | Biochain | 5 slides | EUR 242 |
 Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue II |
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| T8434608 | Biochain | 5 slides | EUR 242 |
 Tissue microarray of top 4 types of cancer (colon |
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| TP484 | TissueArray | each | EUR 198 |
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Description: Tissue microarray of top 4 types of cancer (colon, breast, prostate and lung) and normal tissue, 24 cases/48 cores |
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 Paraffin Tissue Section Panel - Rat Normal Tissue, Multi-tissue III |
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| T8434423 | Biochain | 5 slides | EUR 242 |
 Stomach carcinoma with matched stomach tissue microarray |
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| ST2084b | TissueArray | each | EUR 546 |
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Description: Stomach carcinoma with matched stomach tissue microarray, containing 94 cases of adenocarcinoma, 1 each of signet ring cell carcinoma and squamous cell carcinoma, duplicated cores per case |
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 Normal Tissue, Multi-tissue I) Paraffin Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue I |
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| T8534448 | Biochain | 5 slides | EUR 242 |
 Normal human tissue microarray from 12 organs from autopsy |
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| BN482 | TissueArray | each | EUR 234 |
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Description: Normal human tissue microarray from 12 organs from autopsy, 24 cases/48 cores |
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 Normal Tissue, Multi-tissue III) Paraffin Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue III |
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| T8534423 | Biochain | 5 slides | EUR 242 |
 IV) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) IV |
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| T8234601 | Biochain | 5 slides | EUR 242 |
 V) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) V |
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| T8234602 | Biochain | 5 slides | EUR 242 |
 VI) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VI |
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| T8234603 | Biochain | 5 slides | EUR 242 |
 IX) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) IX |
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| T8234606 | Biochain | 5 slides | EUR 229 |
 X) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) X |
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| T8234607 | Biochain | 5 slides | EUR 229 |
 tissue microarray) Skin cancer and normal tissue high density (69 cases/208 core) tissue microarray |
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| SK208 | TissueArray | each | EUR 546 |
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Description: Skin cancer and normal tissue high density (69 cases/208 core) tissue microarray |
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 Normal Tissue, Multi-tissue II) Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue II |
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| T8534608-Cy | Biochain | 5 slides | EUR 242 |
 VII) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VII |
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| T8234604 | Biochain | 5 slides | EUR 242 |
 VIII) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) VIII |
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| T8234605 | Biochain | 5 slides | EUR 229 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel I, 5 tissue spots |
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| T8235417 | Biochain | 5 slides | EUR 363 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel II, 5 tissue spots |
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| T8235418 | Biochain | 5 slides | EUR 363 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel III, 5 tissue spots |
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| T8235419 | Biochain | 5 slides | EUR 363 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel IV, 5 tissue spots |
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| T8235420 | Biochain | 5 slides | EUR 363 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 5 tissue spots |
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| T8235421 | Biochain | 5 slides | EUR 363 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VI, 5 tissue spots |
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| T8235422 | Biochain | 5 slides | EUR 363 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel I, 8 tissue spots |
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| T8235437 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel II, 8 tissue spots |
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| T8235438 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel III, 8 tissue spots |
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| T8235439 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel IV, 8 tissue spots |
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| T8235440 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 8 tissue spots |
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| T8235441 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VI, 8 tissue spots |
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| T8235442 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VII, 8 tissue spots |
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| T8235443 | Biochain | 5 slides | EUR 565 |
 Paraffin Tissue Section Panel - Human Adult Normal Tissue, Brain I, 7 different tissues |
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| T8234444 | Biochain | 5 slides | EUR 242 |
 Paraffin Tissue Section Panel - Human Adult Normal Tissue, Brain II, 7 different tissues |
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| T8234445 | Biochain | 5 slides | EUR 242 |
 Paraffin Tissue Section Panel - Human Adult Normal Tissue, Brain IV, 7 different tissues |
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| T8234564 | Biochain | 5 slides | EUR 242 |
 I: Major Organ) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) I: Major Organ |
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| T8234431 | Biochain | 5 slides | EUR 242 |
 II: Major Organ) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) II: Major Organ |
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| T8234432 | Biochain | 5 slides | EUR 242 |
 III: Major Organ) Paraffin Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue (8) III: Major Organ |
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| T8234433 | Biochain | 5 slides | EUR 242 |
 Normal Tissue, Multi-tissue I, 8 different tissues) Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue I, 8 different tissues |
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| T8534448-Cy | Biochain | 5 slides | EUR 242 |
 Normal Tissue, Multi-tissue III, 8 different tissues) Paraffin Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue III, 8 different tissues |
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| T8534423-Cy | Biochain | 5 slides | EUR 242 |
 Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue I, 8 different tissues |
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| T8236444Alz | Biochain | 5 slides | EUR 648 |
 Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue II, 7 different tissues |
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| T8236445Alz | Biochain | 5 slides | EUR 648 |
 Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue IV, 7 different tissues |
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| T8236564Alz | Biochain | 5 slides | EUR 648 |
) Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235149 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235152 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235183 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235206 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235248 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235274 | Biochain | 5 slides | EUR 405 |
) Paraffin Tissue Section Panel - Human Tumor Same Tumor Type, Different Donors (5 tissues) |
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| T8235086 | Biochain | 5 slides | EUR 378 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235090 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235106 | Biochain | 5 slides | EUR 405 |
Paraffin Tissue Section Panel - Human Tumor, Same Tumor Type, Different Donors (5 tissues) |
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| T8235142 | Biochain | 5 slides | EUR 405 |
 tissue microarray of astrocytoma) Brain primary tumor high density (69 cases/208 cores) tissue microarray of astrocytoma |
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| GL208 | TissueArray | each | EUR 546 |
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Description: Brain primary tumor high density (69 cases/208 cores) tissue microarray of astrocytoma, glioblastoma, glioblastoma multiforme (GBM) and normal tissue |
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 Paraffin Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues |
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| T8236446Alz | Biochain | 5 slides | EUR 648 |
 Paraffin Tissue Section - Human Brain Tumor: Oligodendroglioma and Astrocytoma, Mixed Glioma |
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| T2235035-12 | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Lupus: Pancreas |
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| T2236188Lup | Biochain | 5 slides | EUR 243 |
 Paraffin Tissue Section - Human Pancreas Tumor |
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| T2235188 | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Diabetic Diseased Tissue: Pancreas |
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| T2236188Dia | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - NASH: Liver |
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| T2236149NAS | Biochain | 5 slides | EUR 524 |
 Paraffin Tissue Section - Lupus: Lung |
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| T2236152Lup | Biochain | 5 slides | EUR 243 |
 Paraffin Tissue Section - Lupus: Heart |
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| T2236122Lup | Biochain | 5 slides | EUR 243 |
 Paraffin Tissue Section - Lupus: Liver |
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| T2236149Lup | Biochain | 5 slides | EUR 243 |
 Paraffin Tissue Section - Asthma: Lung |
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| T2236152Ld-1 | Biochain | 5 slides | EUR 475 |
 Paraffin Tissue Section - Lupus: Kidney |
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| T2236142Lup | Biochain | 5 slides | EUR 243 |
 Paraffin Tissue Section - Lupus: Spleen |
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| T2236246Lup | Biochain | 5 slides | EUR 243 |
 Paraffin Tissue Section - Liver Cirrhosis: Pancreas |
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| T2236188Lcs | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Angioma |
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| T2235011 | Biochain | 5 slides | EUR 238 |
) FFPE Tissue DNA Extraction Micro kit -- For extraction DNA from paraffin-fixed tissues (50prep) |
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| FAFFM-050 | Favorgen | 50 preps | EUR 198 |
) FFPE Tissue DNA Extraction Micro kit -- For extraction DNA from paraffin-fixed tissues (100prep) |
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| FAFFM-100 | Favorgen | 100 preps | EUR 268.8 |
 Paraffin Tissue Section - Emphysema: Lung |
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| T2236152Ld-3 | Biochain | 5 slides | EUR 475 |
 Paraffin Tissue Section - Pneumonia: Lung |
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| T2236152Ld-4 | Biochain | 5 slides | EUR 475 |
 Paraffin Tissue Section - Bronchitis: Lung |
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| T2236152Ld-2 | Biochain | 5 slides | EUR 475 |
 384 Well Storage Microarray Plates, V-Shaped Wells |
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| GP712 | GenDepot | 50 Plates | EUR 602.4 |
 384 Well Storage Microarray Plates, Cylindrical Wells |
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| GP711 | GenDepot | 50 Plates | EUR 602.4 |
 Paraffin Tissue Section - Human Diabetic Diseased Tissue: Parathyroid |
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| T2236189Dia | Biochain | 5 slides | EUR 629 |
 Paraffin Tissue Section - Human Adult Normal: Pancreas |
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| T2234188 | Biochain | 5 slides | EUR 185 |
 Paraffin Tissue Section - hypertension: Heart |
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| T2236122Hd-2 | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Diabetic Diseased Tissue: Lung |
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| T2236152Dia | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Lung Tumor |
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| T2235152 | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Skin Tumor |
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| T2235218 | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Bone Tumor |
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| T2235023 | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Diabetic Diseased Tissue: Colon |
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| T2236090Dia | Biochain | 5 slides | EUR 238 |
 Paraffin Tissue Section - Human Diabetic Diseased Tissue: Liver |
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| T2236149Dia | Biochain | 5 slides | EUR 238 |
