Ago2 Antibody, Brachyury Antibody, Cd45Ro Antibody, Cdk5 Antibody, Foxa2 Antibody, Galr1 Antibody, Gerbil, Guinea, Hamster, Helicobacter, Human, Influenza, Insect, Jmjd3 Antibody, Kangaroo, Lkb1 Antibody, Mobp Antibody, Rabbit, Raccoon, Rat, Reindeer, Tbp Antibody, Zeb2 Antibody

Agar capillary clonogenic microassays for cellular immunocytotoxic activities in human leukaemia and lymphoma

Present ideas of immunotherapeutic approaches in leukemias and lymphomas utilizing activated cytotoxic lymphocytes and macrophages are briefly reviewed. Faulty mobile immunocytotoxic actions and results of interleukins and chemotherapeutic medicine thereupon are mentioned. In vitro assays to measure lymphokine-activated killer (LAK) and pure killer (NK) cell actions endure from varied issues, relying on the standard of the endpoints.
Our clonogenic microassay for LAK cell exercise, utilizing agar-containing glass capillaries, avoids among the potential artifacts and affords a number of benefits which can be mentioned. For example the stimulatory impact of low mafosfamide concentrations on the LAK cell exercise versus Okay562 human myeloid leukemia cells is demonstrated. Thus, our clonogenic LAK microassay gives a sound device for preclinical screening of immunomodulatory brokers.

Microarray evaluation reveals ONC201 mediated differential mechanisms of CHOP gene regulation in metastatic and nonmetastatic colorectal most cancers cells

 

The imipramine ONC201 has antiproliferative results in a number of most cancers cell varieties and prompts built-in stress response pathway related to the induction of Injury Inducible Transcript 3 (DDIT3, also called C/EBP homologous protein or CHOP). We investigated the signaling pathways by which ONC201/CHOP crosstalk is regulated in ONC201-treated nonmetastatic and metastatic most cancers cell traces (Dukes’ kind B colorectal adenocarcinoma nonmetastatic SW480 and metastatic LS-174T cells, respectively). Cell proliferation and apoptosis have been evaluated by MTT assays and circulate cytometry, gene expression was assessed by Affymetrix microarray, signaling pathway perturbations have been assessed in silico, and key regulatory proteins have been validated by Western blotting. Not like LS-174T cells, SW480 cells have been immune to ONC201 remedy; Gene Ontology evaluation of differentially expressed genes confirmed that mobile responsiveness to ONC201 remedy additionally differed considerably.

In each ONC201-treated cell traces, CHOP expression was upregulated; nevertheless, its upstream regulatory mechanisms have been perturbed. Though, PERK, ATF6 and IRE1 ER-stress pathways upregulated CHOP in each cell varieties, the Bak/Bax pathway regulated CHOP solely LS-174T cells. Moreover, CHOP RNA splicing profiles diverse between cell traces; these have been additional modified by ONC201 remedy. In conclusion, we delineated the signaling mechanisms by which CHOP expression is regulated in ONC201-treated non-metastatic and metastatic colorectal cell traces. The noticed variations could possibly be associated to mobile plasticity and metabolic

Analysis of the “AMR Direct Stream Chip Equipment” DNA microarray for detecting antimicrobial resistance genes instantly from rectal and nasopharyngeal scientific samples upon ICU admission

 

Introduction: Immediate detection of antibiotic resistance genes in healthcare establishments is of utmost significance in tackling the unfold of multi-drug resistant micro-organisms. We evaluated the Antimicrobial Resistance (AMR) Direct Stream Chip Equipment versus phenotypic screening assays for rectal and nasopharyngeal specimens upon ICU admission.

Strategies: A complete of 184 twin specimens (92 rectal and 92 nasopharyngeal swabs) from 92 sufferers have been collected from 11/2017 to eight/2018. All swabs have been subjected to each AMR and phenotypic exams based on their origin. The diploma of settlement of the 2 strategies was assessed by the kappa coefficient.

Outcomes: The kappa coefficient confirmed good settlement for MRSA, ESBLs, oxacillinases and vancomycin resistance genes (1.000, p<0.01) and superb settlement for mecA-positive CoNS, KPC-carbapenemases and metallo-beta-lactamases (0.870, p<0.01; 0.864, p<0.01; and 0.912, p<0.01, respectively).

Conclusion: The AMR Direct Stream Chip Equipment is a helpful different to phenotypic testing for fast detection of resistance markers.

Microarray-based identification of differentially expressed genes related to andrographolide derivatives-induced resistance in colon and prostate Most cancers cell traces

 

  • Chemoresistance poses a significant hurdle to most cancers remedies. Andrographolide-derived SRJ09 and SRJ23 have been reported to exhibit potent, selective inhibitory actions towards colon and prostate most cancers cells, respectively. On this examine, beforehand developed resistant colon (HCT-116rst09) and prostate (PC-3rst23) most cancers cell traces have been used to elucidate the molecular mechanisms contributing to chemoresistance.
  • Cytotoxic results of SRJ09 and SRJ23 on each parental and resistant cells have been investigated. Cell cycle distributions in HCT-116rst09cells following SRJ09 remedy have been analysed utilizing circulate cytometry. Entire-genome microarray evaluation was carried out on each parental and resistant cells to acquire differential gene expression profiles. Microarray knowledge have been subjected to protein-protein interplay community, practical enrichment, and pathway analyses.
  • Reverse transcription-polymerase chain response (RT-PCR) was used to validate the adjustments in expression ranges of chosen genes. Apart from morphological adjustments, HCT-116rst09cells confirmed 7.0-fold resistance to SRJ09 whereas PC-3rst23 cells displayed a 5.5-fold resistance to SRJ23, as in contrast with their respective parental cells. G0/G1-phase cell cycle arrest was noticed in HCT-116rst09 cells upon SRJ09 remedy. Collectively, 77 and 21 genes have been discovered differentially modulated in HCT-116rst09 and PC-3rst23 cells, respectively.
  • Subsequent bioinformatics evaluation revealed a number of genes related to FGFR4 and PI3K pathways, and most cancers stemness, have been chemoresistance mediators in HCT-116rst09 RT-PCR confirmed the HMOX1 upregulation and ATG12 downregulation protected the PC-3rst23cells from SRJ23 cytotoxicity. In conclusion, acquired chemoresistance to SRJ09 and SRJ23 in colon and prostate most cancers cells, respectively, could possibly be attributed to the alterations within the expression of genes akin to these associated to PI3K and autophagy pathways.

 

expressionpathology
expressionpathology

The distinctive molecular mechanism of diabetic nephropathy: a bioinformatics evaluation of over 250 microarray datasets

Background/goals: Diabetic nephropathy (DN) is without doubt one of the principal causes of end-stage kidney illness worldwide. Rising research have steered that its pathogenesis is distinct from nondiabetic renal ailments in lots of points. Nevertheless, it nonetheless lacks a complete understanding of the distinctive molecular mechanism of DN.

Strategies: A complete of 255 Affymetrix U133 microarray datasets (Affymetrix, Santa Calra, CA, USA) of human glomerular and tubulointerstitial tissues have been collected. The 22 215 Affymetrix identifiers shared by the Human Genome U133 Plus 2.Zero and U133A Array have been extracted to facilitate dataset pooling.

Subsequent, a linear mannequin was constructed and the empirical Bayes technique was used to pick the differentially expressed genes (DEGs) of every kidney illness. Based mostly on these DEG units, the distinctive DEGs of DN have been recognized and additional analyzed utilizing gene ontology and pathway enrichment evaluation. Lastly, the protein-protein interplay networks (PINs) have been constructed and hub genes have been chosen to additional refine the outcomes.

Outcomes: A complete of 129 and 1251 distinctive DEGs have been recognized within the diabetic glomerulus (upregulated n = 83 and downregulated n = 203) and the diabetic tubulointerstitium (upregulated n = 399 and downregulated n = 874), respectively. Enrichment evaluation revealed that the DEGs within the diabetic glomerulus have been considerably related to the extracellular matrix, cell development, regulation of blood coagulation, ldl cholesterol homeostasis, intrinsic apoptotic signaling pathway and renal filtration cell differentiation.

Within the diabetic tubulointerstitium, the considerably enriched organic processes and pathways included metabolism, the superior glycation finish products-receptor for superior glycation finish merchandise signaling pathway in diabetic problems, the epidermal development issue receptor (EGFR) signaling pathway, the FoxO signaling pathway, autophagy and ferroptosis. By setting up PINs, a number of nodes, akin to AGR2, CSNK2A1, EGFR and HSPD1, have been recognized as hub genes, which could play key roles in regulating the event of DN.

Conclusions: Our examine not solely reveals the distinctive molecular mechanism of DN but in addition gives a worthwhile useful resource for biomarker and therapeutic goal discovery. A few of our findings are promising and must be explored in future work.

 

anti-AXL

YF-PA27171 100 ul
EUR 483.6
Description: Rabbit polyclonal to AXL

Anti-AXL Antibody

A00226-2 100ug/vial
EUR 352.8

Anti-AXL antibody

PAab00754 100 ug
EUR 426

anti-AXL (1B3A2)

LF-MA30204 100 ul
EUR 510
Description: Mouse Monoclonal to AXL

anti-AXL (7E10)

LF-MA30273 100 ul
EUR 583.2
Description: Mouse Monoclonal to AXL

anti- AXL antibody

FNab00754 100µg
EUR 606.3
Description: Antibody raised against AXL

Anti-Axl antibody

STJ91791 200 µl
EUR 236.4
Description: Rabbit polyclonal to Axl.

Anti-Axl antibody

STJ97643 200 µl
EUR 236.4
Description: Rabbit polyclonal to Axl.

Anti-Axl antibody

STJ97856 100 µl
EUR 280.8
Description: Mouse monoclonal to Axl.

Anti-Axl antibody

STJ97857 100 µl
EUR 280.8
Description: Mouse monoclonal to Axl.

Anti-Axl Antibody

STJ500198 100 µg
EUR 571.2

Anti-AXL antibody

STJ119885 100 µl
EUR 332.4
Description: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Anti-AXL antibody

STJ22748 100 µl
EUR 332.4
Description: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Anti-AXL (6C8)

YF-MA10084 100 ug
EUR 435.6
Description: Mouse monoclonal to AXL

Anti-AXL (6G1)

YF-MA10085 100 ug
EUR 435.6
Description: Mouse monoclonal to AXL

Anti-Axl (2C10)

YF-MA12092 100 ug
EUR 435.6
Description: Mouse monoclonal to Axl

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Anti-Axl Antibody BIOTIN

STJ500199 100 µg
EUR 703.2

Anti-Axl Antibody FITC

STJ500200 100 µg
EUR 703.2

Rabbit Anti-Mouse AXL protein IgG-Biotinylated

AXL11-BTN 100 ul
EUR 634.8

Mouse AXL shRNA Plasmid

20-abx973661
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse AXL ELISA Kit

LF-EK50868 1×96T
EUR 777.6

Polyclonal Rabbit Anti-AXL antibody

APR05486G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rabbit Anti-AXL . This antibody is tested and proven to work in the following applications:

Anti-Phospho-AXL-Y702 antibody

STJ11100989 100 µl
EUR 471.6
Description: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Anti-Phospho-Axl (Y691) antibody

STJ90939 200 µl
EUR 236.4
Description: Axl is a protein encoded by the AXL gene which is approximately 98,3 kDa. Axl is localised to the cell membrane. It is involved in the GPCR pathway, ERK signalling, actin nucleation by ARP-WASP complex and activation of cAMP-dependent PKA. This protein falls under the Tyro3-Axl-Mer receptor tyrosine kinase subfamily. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6. Axl is expressed in primary colon tumors but weakly expressed in normal colon tissue. Mutations in the AXL gene may result in lymphocytic choriomeningitis, femoral neuropathy and Borna disease. STJ90939 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Axl protein which only binds about Y691 when Y691 is phosphorylated.

Recombinant AXL Receptor Tyrosine Kinase (AXL)

4-RPL230Hu01
  • EUR 569.44
  • EUR 276.00
  • EUR 1805.38
  • EUR 681.79
  • EUR 1243.58
  • EUR 456.00
  • EUR 4333.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human AXL Receptor Tyrosine Kinase expressed in: E.coli

Recombinant AXL Receptor Tyrosine Kinase (AXL)

4-RPL230Ra01
  • EUR 614.59
  • EUR 288.00
  • EUR 1974.72
  • EUR 738.24
  • EUR 1356.48
  • EUR 487.20
  • EUR 4756.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat AXL Receptor Tyrosine Kinase expressed in: E.coli

AXL antibody

70R-51253 100 ul
EUR 344.4
Description: Purified Polyclonal AXL antibody

Axl Antibody

39365-100ul 100ul
EUR 468

AXL Antibody

48205-100ul 100ul
EUR 399.6

AXL Antibody

48205-50ul 50ul
EUR 286.8

AXL antibody

10R-1995 100 ul
EUR 392.4
Description: Mouse monoclonal AXL antibody

AXL antibody

10R-2051 100 ul
EUR 483.6
Description: Mouse monoclonal AXL antibody

AXL Antibody

ABF8412 100 ug
EUR 525.6

AXL siRNA

20-abx908739
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

AXL siRNA

20-abx908740
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

AXL antibody

70R-9655 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal AXL antibody

AXL Antibody

AF6824 100ul
EUR 420

AXL Antibody

AF7793 200ul
EUR 540

AXL Antibody

AF8412 200ul
EUR 540

AXL Antibody

BF0290 200ul
EUR 540

AXL Antibody

1-CSB-PA030245
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against AXL. Recognizes AXL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000

AXL Antibody

1-CSB-PA297707
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against AXL. Recognizes AXL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

AXL Antibody

1-CSB-PA326981LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against AXL. Recognizes AXL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

AXL Antibody

F50552-0.08ML 0.08 ml
EUR 165

AXL Antibody

F50552-0.4ML 0.4 ml
EUR 379

AXL Antibody

F50553-0.08ML 0.08 ml
EUR 165

AXL Antibody

F50553-0.4ML 0.4 ml
EUR 379

AXL Antibody

F50554-0.08ML 0.08 ml
EUR 165

AXL Antibody

F50554-0.4ML 0.4 ml
EUR 379

AXL Antibody

F53357-0.08ML 0.08 ml
EUR 165

AXL Antibody

F53357-0.4ML 0.4 ml
EUR 379

AXL Antibody

F44438-0.08ML 0.08 ml
EUR 165

AXL Antibody

F44438-0.4ML 0.4 ml
EUR 379

AXL Antibody

RQ6041 100 ug
EUR 419
Description: Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. This gene is mapped to 19q13.2. The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Rabbit Anti-Mouse AXL protein IgG, aff pure

AXL11-A 100 ul
EUR 578.4

Mouse Monoclonal Anti-Human AXL protein IgG-Biotinylated

AXL13-BTN 100 ul
EUR 634.8

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

AXL ELISA Kit (Mouse) (OKBB00412)

OKBB00412 96 Wells
EUR 606
Description: Description of target: Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Mouse AXL PicoKine ELISA Kit

EK0660 96 wells
EUR 510
Description: For quantitative detection of mouse AXL in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Axl ORF Vector (Mouse) (pORF)

ORF039463 1.0 ug DNA
EUR 607.2

Axl ORF Vector (Mouse) (pORF)

ORF039464 1.0 ug DNA
EUR 607.2

Axl ORF Vector (Mouse) (pORF)

ORF039465 1.0 ug DNA
EUR 607.2

ELISA kit for Mouse AXL

EK5264 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse AXL in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse Monoclonal Anti-Human AXL protein IgG, aff pure

AXL13-M 100 ul
EUR 578.4

AXL Receptor Tyrosine Kinase (AXL) Polyclonal Antibody (Rat)

4-PAL230Ra01
  • EUR 330.00
  • EUR 3549.60
  • EUR 872.40
  • EUR 420.00
  • EUR 271.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat AXL Receptor Tyrosine Kinase (AXL)

Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

SEL230Hu-10x96wellstestplate 10x96-wells test plate
EUR 6227.58
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human AXL Receptor Tyrosine Kinase (AXL) in serum, plasma, tissue homogenates and other biological fluids.

Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

SEL230Hu-1x48wellstestplate 1x48-wells test plate
EUR 618.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human AXL Receptor Tyrosine Kinase (AXL) in serum, plasma, tissue homogenates and other biological fluids.

Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

SEL230Hu-1x96wellstestplate 1x96-wells test plate
EUR 831.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human AXL Receptor Tyrosine Kinase (AXL) in serum, plasma, tissue homogenates and other biological fluids.

Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

SEL230Hu-5x96wellstestplate 5x96-wells test plate
EUR 3381.66
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human AXL Receptor Tyrosine Kinase (AXL) in serum, plasma, tissue homogenates and other biological fluids.

Human AXL Receptor Tyrosine Kinase (AXL) ELISA Kit

4-SEL230Hu
  • EUR 6288.00
  • EUR 3322.80
  • EUR 831.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human AXL Receptor Tyrosine Kinase (AXL) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.