Ago2 Antibody, Brachyury Antibody, Brn3A Antibody, Cd45Ro Antibody, Cdk5 Antibody, Erg Antibody, Foxa2 Antibody, Guinea, Hamster, Helicobacter, Horse, Human, Lkb1 Antibody, Parp1 Antibody, Pig, Raccoon, Rat, Reindeer, Reptile, Zeb2 Antibody

Mass spectrometry-based microassay of (2)H and (13)C plasma glucose labeling to quantify liver metabolic fluxes in vivo

Mouse fashions designed to look at hepatic metabolism are essential to diabetes and weight problems analysis. Thus, a microscale methodology to quantitatively assess hepatic glucose and middleman metabolism in aware, unrestrained mice was developed. [(13)C3]propionate, [(2)H2]water, and [6,6-(2)H2]glucose isotopes have been delivered intravenously in short- (9 h) and long-term-fasted (19 h) C57BL/6J mice. GC-MS and mass isotopomer distribution (MID) evaluation have been carried out on three 40-μl arterial plasma glucose samples obtained throughout the euglycemic isotopic regular state. Mannequin-based regression of hepatic glucose and citric acid cycle (CAC)-related fluxes was carried out utilizing a complete isotopomer mannequin to trace carbon and hydrogen atom transitions via the community and thereby simulate the MIDs of measured fragment ions.

Glucose-6-phosphate manufacturing from glycogen diminished, and endogenous glucose manufacturing was completely gluconeogenic with extended fasting. Gluconeogenic flux from phosphoenolpyruvate (PEP) remained steady, whereas that from glycerol modestly elevated from short- to long-term fasting. CAC flux [i.e., citrate synthase (VCS)] was lowered with long-term fasting. Apparently, anaplerosis and cataplerosis elevated with quick length; accordingly, pyruvate carboxylation and the conversion of oxaloacetate to PEP have been severalfold increased than VCS in long-term fasted mice.

This methodology makes use of state-of-the-art in vivo methodology and complete isotopomer modeling to quantify hepatic glucose and middleman fluxes throughout physiological stress in mice. The small plasma necessities allow serial sampling with out stress and the affirmation of steady-state glucose kinetics. Moreover, the method can accommodate a broad vary of modeling assumptions, isotope tracers, and measurement inputs with out the necessity to introduce advert hoc mathematical approximations.

 

Spectrophotometric exercise microassay for pure and recombinant cytochrome P450-type nitric oxide reductase

Nitric oxide reductase (NOR) of the P450 oxidoreductase household accepts electrons straight from its cofactor, NADH, to cut back two nitric oxide (NO) molecules to at least one nitrous oxide molecule and water. The enzyme performs a key function within the elimination of radical NO produced throughout respiratory metabolism, and purposes in bioremediation and biocatalysis have been recognized. Nonetheless, a speedy, correct, and delicate enzyme assay has not but been developed for this enzyme household.
On this examine, we optimized response situations for the event of a spectrophotometric NOR exercise microassay utilizing NOC-5 for the supply of NO in resolution. We additionally reveal that the assay is appropriate for the quantification and characterization of P450-type NOR. The Okay(m) and ok(cat) kinetic constants obtained by this assay have been corresponding to the values decided by gasoline chromatography, however with improved comfort and price effectivity, successfully by miniaturization. To our data, that is the primary examine to current the quantification of NOR exercise in a kinetic microassay format.

Densitometry based mostly microassay for the willpower of lipase depolymerizing exercise on polyhydroxyalkanoate.

A novel methodology for the assay of polyhydroxyalkanoate (PHA)-degrading potential of triacylglycerol lipases was developed. By making use of the pure affinity of lipases in the direction of hydrophobic interfaces, a delicate and speedy densitometry evaluation for the analysis of hydrolytic exercise of lipase droplets in the direction of PHA-coated floor was efficiently carried out. We discovered that 12 out of 14 examined lipases that are of fungal, bacterial and animal origin have been in a position to hydrolyze P(3HB-co-92 mol% 4HB) skinny movie.
The patterns and opacity of the hydrolysis spots of lipases on PHA movies allowed simple comparability of PHA-hydrolytic power of lipases. Lipase from the bacterium Chromobacterium viscosum exhibited the very best PHA-degrading exercise. The hydrolytic exercise of lipases on water insoluble PHA, emulsified p-nitrophenyl laurate and olive oil have been additionally in contrast and apparently some lipases confirmed higher exercise when PHA was used as a substrate.

A semiautomatic microassay to measure human tumor clonogenic growth-invitro

A semi-automatic micromethod was developed utilizing 96-well microtiter plates together with a video monitoring system to assay human tumor clonogenic progress in vitro. The situations to comply with the expansion of Okay562 human myeloid colonies have been optimized and validated: About 250 cells in a 40 mul agar combination are to be seeded per nicely. The colony quantity is proportional to the variety of seeded cells. The cloning effectivity is about 75% and correlates nicely with the established glass capillary methodology.
The system precision of the automated colony counter, expressed by the coefficient of variation, is lower than 2%. The imply of the intra- and inter assay coefficient of variation is low (8.5% and seven%, respectively). The microassay was utilized to measure the lymphokine-activated killer (LAK)-activity of peripheral blood mononuclear cells (PBMC) from wholesome donors in opposition to Okay562 goal cells and to look at the results of varied thymic hormone preparations on the cytotoxicity of generated LAK cells. Thus, the brand new microassay gives a useful gizmo for measuring clonogenic tumor cell progress and its modulation by totally different brokers on many samples in a short while.
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Physiological microassay of plasma complete antioxidant standing in a mannequin of endothelial dysfunction within the rat following experimental oxidant stress in vivo

We’ve got developed a photometric microassay for the evaluation of complete antioxidant standing in plasma at physiological pH and temperature and utilized it to guage experimental oxidant stress in vivo related to endothelial dysfunction in vitro. Rat plasma or l-ascorbic acid inhibited the peroxidase-mediated accumulation after 6 min at pH 7.four and 37°C of ABTS(+) (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical), measured at 405 nm, in a concentration-dependent method.
Plasma complete antioxidant standing, expressed because the ascorbate equal antioxidant focus, was subsequently discovered to be considerably lowered in rats handled each day for 7 days in vivo with the oxidant compounds hydroquinone (50 mg/kg i.p.) and triethylenetetramine (100 mg/kg i.p.), both alone or together with the glutathione-depleting agent l-buthionine sulfoximine (50 mg/kg i.p). Moreover, basal endothelial operate in remoted aorta was impaired after hydroquinone or triethylenetetramine in a fashion aggravated by l-buthionine sulfoximine.

The methylene blue colorimetric microassay for figuring out cell line response to progress components.

The validity of the methylene blue colorimetric microassay for figuring out the response of monolayers of human ovarian tumour cell traces to totally different progress components was investigated. Linearity of the connection between cell density and optical density was confirmed for every cell line (r=0.989-0.999,p<0.001), and when preliminary cell density was optimised to offer exponential progress over the assay interval, variations in response to medium dietary supplements have been apparent.
The response of goal cells to progress components, obtained utilizing the methylene blue assay, have been in contrast with, and located to parallel, beforehand documented responses obtained non-colorimetrically. Thus Mink lung epithelial cells (MLEC) have been inhibited by TGβ (Holleyet al., 1983), EGF had an inhibitory impact on A431 cells (Gill & Lazar, 1981; Barnes, 1982), and the mesothelial cell line confirmed a proliferative response to EGF and hydrocortisone (Connell and Rheinwald, 1983).The methylene blue colorimetric microssay was discovered to be a easy, dependable, delicate methodology with low variability, for figuring out the response of cultured cells to progress components.

Frozen Tissue Section Panel - Mouse Normal Tissue, Multi-tissue I

T6334447 5 slides
EUR 504

Frozen Tissue Section Panel - Mouse Normal Tissue, Multi-tissue II

T6334608 5 slides
EUR 504

Frozen Tissue Section Panel - Mouse Normal Tissue, Multi-tissue III

T6334423 5 slides
EUR 504

High density tissue microarray of Hodgkin's Disease

LM208 each
EUR 546
Description: High density tissue microarray of Hodgkin's Disease, Non-Hodgkin's lymphoma and normal lymph node tissues, 69 cases/208 cores

Tissue microarray of top 4 types of cancer (colon

TP484 each
EUR 198
Description: Tissue microarray of top 4 types of cancer (colon, breast, prostate and lung) and normal tissue, 24 cases/48 cores

Stomach carcinoma with matched stomach tissue microarray

ST2084b each
EUR 546
Description: Stomach carcinoma with matched stomach tissue microarray, containing 94 cases of adenocarcinoma, 1 each of signet ring cell carcinoma and squamous cell carcinoma, duplicated cores per case

Skin cancer and normal tissue high density (69 cases/208 core) tissue microarray

SK208 each
EUR 546
Description: Skin cancer and normal tissue high density (69 cases/208 core) tissue microarray

Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue I

T6434448 5 slides
EUR 504

Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue III

T6434423 5 slides
EUR 504

Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue I

T6234431 5 slides
EUR 507

Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue II

T6234432 5 slides
EUR 507

Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue III

T6234433 5 slides
EUR 507

Frozen Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue I

T6534448 5 slides
EUR 504

Frozen Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue III

T6534423 5 slides
EUR 504

Frozen Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue I

T6534448-Cy 5 slides
EUR 504

Frozen Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue III

T6534423-Cy 5 slides
EUR 504

Brain primary tumor high density (69 cases/208 cores) tissue microarray of astrocytoma

GL208 each
EUR 546
Description: Brain primary tumor high density (69 cases/208 cores) tissue microarray of astrocytoma, glioblastoma, glioblastoma multiforme (GBM) and normal tissue

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue I, 7 different tissues

T6236444Alz 5 slides
EUR 1135

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue II, 7 different tissues

T6236445Alz 5 slides
EUR 1135

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues

T6236446Alz 5 slides
EUR 1135

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue IV, 7 different tissues

T6236564Alz 5 slides
EUR 1135

384 Well Storage Microarray Plates, Cylindrical Wells

GP711 50 Plates
EUR 602.4

384 Well Storage Microarray Plates, V-Shaped Wells

GP712 50 Plates
EUR 602.4

Frozen Tissue Section - Lupus: Pancreas

T1236188Lup 5 slides
EUR 515

FDA Standard Frozen Tissue Array - Mouse Normal

T6334701-1 2 slides
EUR 629

FDA Standard Frozen Tissue Array - Mouse Normal

T6334701-2 4 slides
EUR 1135

Frozen Tissue Section - Human Diabetic Diseased Tissue: Pancreas

T1236188Dia 5 slides
EUR 475

Frozen Tissue Section - Human Tumor: Pancreas

T1235188 5 slides
EUR 330

Rabbit MUSCLE TISSUE MT FROZEN 1kg

41325-2 1 kg
EUR 337.69

Frozen Tissue Section - Asthma: Lung

T1236152Ld-1 5 slides
EUR 475

Frozen Tissue Section - Lupus: Ovary

T1236183Lup 5 slides
EUR 682

Frozen Tissue Section - Lupus: Kidney

T1236142Lup 5 slides
EUR 587

Frozen Tissue Section - Lupus: Spleen

T1236246Lup 5 slides
EUR 515

Frozen Tissue Section - Lupus: Adrenal

T1236004Lup 5 slides
EUR 515

Frozen Tissue Section - Emphysema: Lung

T1236152Ld-3 5 slides
EUR 475

Frozen Tissue Section - Pneumonia: Lung

T1236152Ld-4 5 slides
EUR 475

Frozen Tissue Section - Bronchitis: Lung

T1236152Ld-2 5 slides
EUR 475

Frozen Tissue Section - hypertension: Vein

T1236020Hd-2 5 slides
EUR 475

Frozen Tissue Section - hypertension: Heart

T1236122Hd-2 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Eye

T1236108Dia 5 slides
EUR 662

Frozen Tissue Section - hypertension: Artery

T1236013Hd-2 5 slides
EUR 475

Frozen Tissue Section - hypertension: Kidney

T1236142Hd-2 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Lung

T1236152Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Skin

T1236218Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Brain

T1236035Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Colon

T1236090Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Heart

T1236122Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Liver

T1236149Dia 5 slides
EUR 475

Frozen Tissue Array - Human Adult Normal Tissue, Multi-tissue I

T6234700-1 1 slide
EUR 265

Frozen Tissue Array - Human Adult Normal Tissue, Multi-tissue I

T6234700-5 5 slides
EUR 1004

Frozen Tissue Section - Human Diabetic Diseased Tissue: Kidney

T1236142Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Spleen

T1236246Dia 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Pancreas

T1234188 5 slides
EUR 343

Frozen Tissue Section - Human Diabetic Diseased Tissue: Adrenal

T1236004Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Stomach

T1236248Dia 5 slides
EUR 475

Frozen Tissue Section - Human Tumor: Bone

T1235023 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Lung

T1235152 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Skin

T1235218 5 slides
EUR 330

Frozen Tissue Section - Arteriosclerosis: Aorta

T1236012Hd-4 5 slides
EUR 1060

Frozen Tissue Section - Human Tumor: Brain

T1235035 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Colon

T1235090 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Liver

T1235149 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Ovary

T1235183 5 slides
EUR 330

Frozen Tissue Section - Lupus: Spinal Cord

T1236234Lup 5 slides
EUR 515

Frozen Tissue Section - Arteriosclerosis: Artery

T1236013Hd-4 5 slides
EUR 1060

Frozen Tissue Section - Human Diabetic Diseased Tissue: Esophagus

T1236106Dia 5 slides
EUR 475

Frozen Tissue Section - Human Tumor: Breast

T1235086 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Kidney

T1235142 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Rectum

T1235206 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Testis

T1235260 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Adrenal

T1235004 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Bladder

T1235010 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Parotid

T1235190 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Stomach

T1235248 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Thymoma

T1235264 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Thyroid

T1235265 5 slides
EUR 330

Frozen Tissue Array - Dog Male Adult Normal Tissue, Multi-tissue I

T6734700-G01-1 1 slide
EUR 239

Frozen Tissue Array - Dog Male Adult Normal Tissue, Multi-tissue I

T6734700-G01-5 5 slides
EUR 883

Frozen Tissue Section - Human Tumor: Lymphoma

T1235161 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Prostate

T1235201 5 slides
EUR 330

Multiple Species Frozen Tissue Array - Brain

T6134035-1 1 slide
EUR 195

Multiple Species Frozen Tissue Array - Brain

T6134035-5 5 slides
EUR 711

Multiple Species Frozen Tissue Array - Liver

T6134149-1 1 slide
EUR 195

Multiple Species Frozen Tissue Array - Liver

T6134149-5 5 slides
EUR 711

Frozen Tissue Array - Human Tumor and Normal Tissue, Multi-tissue I

T6235700-1 1 slide
EUR 415

Frozen Tissue Array - Human Tumor and Normal Tissue, Multi-tissue I

T6235700-5 5 slides
EUR 1280

Frozen Tissue Section - Human Tumor: Esophagus

T1235106 5 slides
EUR 330

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-5 5 slides
EUR 883

Frozen Tissue Array - Rabbit Male Adult Normal Tissue, Multi-tissue I

T6834700-G01-1 1 slide
EUR 239