Expression Pathology

Liquid Tissue® MS Protein Prep Kit - for discovery, validation and targeted tissue proteomic assays in FFPE tissue

Instruction Manual | Product Data Sheet | Application Notes | References

The patented process used in the Liquid Tissue® MS Protein Prep Kit enables mass spectrometry-based quantitation and discovery of protein biomarkers in archival tissue. It is fully compatible with multiple reaction monitoring (MRM) based quantitation. Download the case study on Her2 in invasive breast cancer here.

A protocol is available to detect differential protein expression using iTRAQ™** labeling. Download here.

A standard preparation uses a lot less tissue than any other published methods. The Liquid Tissue® MS Protein Prep Kit only needs 30,000 cells, the equivalent of a 2 mm x 4 mm region from a 10 µm thick section of FFPE tissue, and will yield 5-10 µg of total protein.

Download the most recent revision of the instruction manual.

ORDERING INFORMATION
Catalog Number	Product Description
10001-010	Liquid Tissue® MS Protein Prep Kit, 4 preparations
10001-023	Liquid Tissue® MS Protein Prep Kit, 10 preparations
10001-053	Liquid Tissue® MS Protein Prep Kit, 50 preparations
Inquire for price and availability for the larger package sizes.

Our Terms and Conditions apply. The Liquid Tissue® MS is sold under a limited license with commercial use restrictions. For further details please contact us.

Samples processed using the Liquid Tissue® MS Protein Prep Kit are not suitable for analysis using gel electrophoresis or for printing reverse-phase protein arrays.

DIRECTOR® Laser Microdissection Slides—Direct non-contact microdissection without plastic film or sticky caps

Fast Forward Microdissection - works, without modifications, with the three leading laser microdissection platforms; the Leica LMD6000, the Zeiss P.A.L.M. Microbeam and the new mmi CellCut Plus from Molecular Machines & Industries.

Watch the videos showing the principles and the slides in use

Product Data Sheet | Application Notes | References

The patented DIRECTOR slides feature an energy transfer coating that replaces plastic films or adhesives for direct non-contact microdissection. This makes possible fast and precise harvesting of even the most specific cellular features, from fine, subcellular features to regions of tissue.

DIRECTOR slides are compatible with commonly used gene and protein expression analysis methods, and work with tissues or cells, whether fresh, frozen or fixed (inc. standard formalin fixed paraffin embedded (FFPE) tissue).

The slides are inert to a wide range of chemical and thermal conditions, and can be autoclaved, and are stable in organic solvents such as xylenes. DIRECTOR slides will not epifluoresce, making them ideally suited for applications requiring fluorescent stains, as well as those applications requiring DIC or polarized light. Tissue sections will adhere to the DIRECTOR slides just as well as to any standard pathology glass slides, without pretreatment.

The DIRECTOR slides are based on Laser Induced Forward Transfer (LIFT), a non-contact microdissection technology utilizing a thin energy transfer coating. Laser energy is transferred to the transfer coating, the coating is vaporized and the selected feature instantly shot into the collection tube. You collect exactly what you see in the microscope. The energy transfer coating absorbs the laser energy, so the biomolecules in the sample are not affected. Since the coating is completely vaporized there is absolutely no contamination of your sample.

ORDERING INFORMATION
Catalog Number	Product Description
50001-018	DIRECTOR® Laser Microdissection Slides, 10 slides
50001-024	DIRECTOR® Laser Microdissection Slides, 50 slides
Volume discounts available.

ProteoSOL™ Tissue Extraction System

Available from KPL, Kirkegaard & Perry Laboratories, the ProteoSOL™ Tissue Extraction System is based on the Liquid Tissue® ID Protein Prep Kit. It is designed for the extraction of proteins directly from formalin fixed paraffin-embedded (FFPE) tissue samples. The system enables immunodetection of proteins in a membrane-based assay. Samples processed with this system can be arrayed and detected on a membrane using high-throughput detection methods. Immunodetection with specific antibodies allows the direct comparison of a specific protein across multiple samples in a single array.

*Please note that samples processed using the ProteoSOL Tissue Extraction System are not suitable for analysis using mass spectrometry or gel electrophoresis.

, *Thermo Finnigan Proteome X LTQ Mass Spectrometer .** iTRAQ is a registered trademark of Applied Biosystems.

Liquid Tissue technology is protected by U.S. Patent 7,473,532 and patents pending and foreign equivalents thereof.

DIRECTOR technology is protected by U.S. Patents 7,294,367 and 7,381,440 and foreign equivalents thereof.

Our Terms and Conditions apply. The Liquid Tissue® MS is sold under a limited license with commercial use restrictions. For further details please contact us.

Expression Pathology Inc. is a leader in tissue proteomics.

Using our proprietary tissue analysis platform we are developing targeted tissue proteomic assays with a focus on oncology. The tests will provide information to guide treatment decisions – the presence of protein drug targets, or which biological pathways are activated in tumors to aid prognosis and drug selection.

Our scientists have extensive experience in pathology, proteomics and tissue analysis. We can help you design your experiment, and we can also help you with grant writing.

TO ORDER

Call us to order or discuss projects (+1.301.977.3654), American Eastern Time, 9AM to 5PM. We accept VISA and MasterCard, and purchase orders from the United States and Canada.

Distributors and Representatives

Japan: AMR Inc.

Korea: Kim & Friends, Inc.

Singapore, Malaysia, Thailand, and Vietnam: ChemoResearch Pte. Ltd.

Taiwan: GenDiscovery

Please inquire for quotations and shipping details to other parts of the world.

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News & Events

Expression Pathology Collaborates with Toronto’s Sick Kids Hospital and University Health Network on New Mass Spec EGFR Test, May 4, 2010, (press release)
Thermo Fisher announced a collaboration with Expression Pathology to develop Liquid Tissue MRM Assays of Cancer Proteins In Microdissected FFPE Tissue, December 16, 2009 (press release)
The Mayo Clinic has licensed non-exclusive rights to the Liquid Tissue(R) patent for use in diagnosis of systemic amyloidosis in FFPE tissue, November 17, 2009 (press release)
Expression Pathology raises $6.5 Million to develop personalized medicine assays for protein biomarkers in FFPE Tissue. April 20, 2009 (press release)